Human papillomavirus DNA in cervical carcinoma—correlation with clinical data and influence on prognosis

Abstract

Human papillomavirus (HPV) is a main factor in cervical carcinogenesis. However, data on the correlation of HPV with clinical features and the prognosis of cervical carcinoma remain controversial. The HPV status (positivity, type, copy number) in unfixed tissue specimens of 205 primary invasive cervical carcinomas was determined by Southern blot hybridization. A correlation with comprehensive clinical and histopathologic data and long-time survival was evaluated. HPV DNA was detected in 73% of the cases; 83% of the HPV-positive tumors contained HPV 16. HPV 16 was predominant among squamous-cell carcinomas (SCC) (ρ = 0.05). HPV 16 copy number was higher in keratinizing tumors (ρ < 0.05), and elevated levels of the SCC antigen were more common in patients positive for HPV 16 (ρ < 0.03). No association was found between the HPV status and 8 other clinical and histopathologic variables. Multivariate analysis after a median follow-up of 73 months demonstrated longer survival for patients with lower clinical stage (ρ = 0.001) and keratinizing SCC (ρ = 0.005). Women with HPV-negative tumors had a higher risk of death (RR 1.51; ρ = 0.07). HPV analysis does not clearly define biologically distinct sub-sets of cervical carcinoma. This underlines the importance of additional factors in cervical carcinogenesis.