Synthesis of cartilage matrix by mammalian chondrocytes in vitro. I. Isolation, culture characteristics, and morphology.
Open Access
- 1 June 1982
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 93 (3), 743-750
- https://doi.org/10.1083/jcb.93.3.743
Abstract
We describe the isolation and the ultrastructural characteristics of adult bovine articular chondrocytes in vitro. Slices of bovine articular cartilage undergo sequential digestions with pronase and collagenase in order to release cells. Chondrocytes are plated at high density (1 x 10(5) cells/cm2) in culture dishes or roller bottles with Ham's F-12 medium, supplemented with 10% fetal bovine serum. Before culture, chondrocytes are freed of surrounding territorial matrix. Within the first few days of culture they re-establish a territorial matrix. As time progresses, chondrocytes synthesize both territorial and extraterritorial matrices. The matrices are rich in collagen fibrils and ruthenium red-positive proteoglycans. These features are most apparent in mass roller cultures in which aggregates of cells and matrix appear as long streaks and nodules. This morphology reveals an organization of chondrocytes and their matrices that is similar to that of the parent articular cartilage in vivo.Keywords
This publication has 23 references indexed in Scilit:
- Evidence for histogenic interactions during in vitro limb chondrogenesisDevelopmental Biology, 1980
- Cartilage cell differentiation: review.1979
- [50] Large-scale preparation of chondrocytesMethods in Enzymology, 1979
- Electron microscopic and biochemical studies of proteoglycan polydispersity in chick limb bud chondrocyte culturesJournal of Biological Chemistry, 1978
- Stimulation of Chondromucoprotein Synthesis in Chondrocytes by Extracellular ChondromucoproteinProceedings of the National Academy of Sciences of the United States of America, 1972
- Effects of the nicotinamide-sensitive teratogen 3-acetylpyridine on chick limb cells in cultureExperimental Cell Research, 1970
- Sulfate incorporation by articular chondrocytes in monolayer cultureArthritis & Rheumatism, 1970
- THE LOSS OF PHENOTYPIC TRAITS BY DIFFERENTIATED CELLSThe Journal of Experimental Medicine, 1969
- Clonal stability and phenotypic expression of chick cartilage cells in vitro.Proceedings of the National Academy of Sciences of the United States of America, 1966
- Electron Microscope Study of DNA-Containing PlasmsThe Journal of cell biology, 1958