Borealin is differentially expressed in ES cells and is essential for the early development of embryonic cells
- 3 March 2008
- journal article
- research article
- Published by Springer Science and Business Media LLC in Molecular Biology Reports
- Vol. 36 (3), 603-609
- https://doi.org/10.1007/s11033-008-9220-9
Abstract
Maintaining undifferentiated state and self-renewal ability of embryonic stem cells is a process that many genes and factors participate in. Using bioinformatics analyses and suppression subtractive hybridization we cloned a novel human gene related to the proliferation of human embryonic stem (hES) cells and its mouse homologue and identified them as being borealin. Our data demonstrated that borealin was highly expressed in undifferentiated ES cells, mouse pre-implantation embryos and the brain of 8.5–9.5 day post-coitum mouse embryos. Furthermore, following Borealin depletion by microinjecting anti-Borealin antibody into the zygotes the mouse embryos were arrested at the 2 or 4-cell stage and chromosomes could not correctly localize at the equator plane of the mitotic spindle and most cells had two or more nuclei. Taken together, these results indicate that Borealin plays a crucial role in the early mouse embryonic development.Keywords
This publication has 31 references indexed in Scilit:
- Borealin is repressed in response to p53/Rb signalingCell Biology International, 2007
- Chromosomal passengers: the four-dimensional regulation of mitotic eventsChromosoma, 2004
- BorealinThe Journal of cell biology, 2004
- Suppression of survivin expression inhibits in vivo tumorigenicity and angiogenesis in gastric cancer.2003
- Requirement for Foxd3 in maintaining pluripotent cells of the early mouse embryoGenes & Development, 2002
- TheSchizosaccharomyces pombeAurora–related Kinase Ark1 Interacts with the Inner Centromere Protein Pic1 and Mediates Chromosome Segregation and CytokinesisMolecular Biology of the Cell, 2002
- Genomic Organization, Expression, and Chromosome Localization of a Third Aurora-Related Kinase Gene,Aie1DNA and Cell Biology, 2000
- New type of POU domain in germ line-specific protein Oct-4Nature, 1990
- Inhibition of pluripotential embryonic stem cell differentiation by purified polypeptidesNature, 1988
- Establishment in culture of pluripotential cells from mouse embryosNature, 1981