Phospholipid and sterol analysis of plasma membranes of azole-resistantCandida albicansstrains
Open Access
- 1 April 2000
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 185 (1), 59-63
- https://doi.org/10.1111/j.1574-6968.2000.tb09040.x
Abstract
The phospholipid and sterol composition of the plasma membranes of five fluconazole-resistant clinical Candida albicans isolates was compared to that of three fluconazole-sensitive ones. The three azole-sensitive strains tested and four of the five resistant strains did not exhibit any major difference in their phospholipid and sterol composition. The remaining strain (R5) showed a decreased amount of ergosterol and a lower phosphatidylcholine:phosphatidylethanolamine ratio in the plasma membrane. These changes in the plasma membrane lipid and sterol composition may be responsible for an altered uptake of drugs and thus for a reduced intracellular accumulation of fluconazole thereby providing a mechanism for azole resistance.Keywords
This publication has 23 references indexed in Scilit:
- Molecular analysis of cyp51 from fluconazole-resistant Candida albicans strainsFEMS Microbiology Letters, 1997
- The Mutation T315A in Candida albicans Sterol 14α-Demethylase Causes Reduced Enzyme Activity and Fluconazole Resistance through Reduced AffinityPublished by Elsevier BV ,1997
- Mode of Action and Resistance to Azole Antifungals Associated with the Formation of 14α-Methylergosta-8,24(28)-dien-3β,6α-diolBiochemical and Biophysical Research Communications, 1995
- Resistance of Candida albicans to azole antifungal agentsBiochemical Society Transactions, 1993
- A simple method to renature DNA-binding proteins separated by SDS-polyacrylamide gel electrophoresisNucleic Acids Research, 1993
- Cloning and characterization of the plasma membrane H(+)-ATPase from Candida albicansJournal of Bacteriology, 1991
- Molecular Dynamics Simulation of a Phospholipid MicelleScience, 1989
- [40] H+-ATPase from plasma membranes of Saccharomyces cerevisiae and Avena sativa roots: Purification and reconstitutionMethods in enzymology, 1988
- Determinants of membrane protein topology.Proceedings of the National Academy of Sciences of the United States of America, 1987
- The Fluid Mosaic Model of the Structure of Cell MembranesScience, 1972