Isolation, identification and characterization of Bacillus subtilis ZJB-063, a versatile nitrile-converting bacterium
- 24 October 2007
- journal article
- biotechnologically relevant-enzymes-and-proteins
- Published by Springer Science and Business Media LLC in Applied Microbiology and Biotechnology
- Vol. 77 (5), 985-993
- https://doi.org/10.1007/s00253-007-1236-x
Abstract
Strain ZJB-063, a versatile nitrile-amide-degrading strain, was newly isolated from soil in this study. Based on morphology, physiological tests, Biolog and the 16S rDNA sequence, strain ZJB-063 was identified as Bacillus subtilis. ZJB-063 exhibited nitrilase activity without addition of inducers, indicating that the nitrilase in B. subtilis ZJB-063 is constitutive. Interestingly, the strain exhibited nitrile hydratase and amidase activity with the addition of ɛ-caprolactam. Moreover, the substrate spectrum altered with the alteration of enzyme systems due to the addition of ɛ-caprolactam. The constitutive nitrilase was highly specific for arylacetonitriles, while the nitrile hydratase/amidase in B. subtilis ZJB-063 could not only hydrolyze arylacetonitriles but also other nitriles including some aliphatic nitriles and heterocyclic nitriles. Despite comparatively low activity, the amidase of hydratase/amidase system was effective in converting amides to acids. The versatility of this strain in the hydrolysis of various nitriles and amides makes it a potential biocatalyst in organic synthesis.Keywords
This publication has 28 references indexed in Scilit:
- Isolation of glycolonitrile-hydrolyzing microorganism based on colorimetric reactionEnzyme and Microbial Technology, 2007
- Isolation and identification of a novel Rhodococcus sp. ML-0004 producing epoxide hydrolase and optimization of enzyme productionProcess Biochemistry, 2007
- Directed Evolution and Characterization of a Novel d-Pantonohydrolase from Fusarium moniliformeJournal of Agricultural and Food Chemistry, 2006
- Nocardia globerulaNHB-2: a versatile nitrile-degrading organismCanadian Journal of Microbiology, 2005
- Cloning and expression of d -lactonohydrolase cDNA from Fusarium moniliforme in Saccharomyces cerevisiaeBiotechnology Letters, 2004
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994
- Enzymatic synthesis of acrylamide: a success story not yet overTrends in Biotechnology, 1992
- A novel nitrilase, arylacetonitrilase, of Alcaligenes faecalis JM3European Journal of Biochemistry, 1990
- Nitrilase of Rhodococcus rhodochrous J1European Journal of Biochemistry, 1989
- Occurrence of a cobalt-induced and cobalt-containing nitrile hydratase in RhodococcusRhodochrous J1Biochemical and Biophysical Research Communications, 1988