Melatonin protects against endosulfan‐induced oxidative tissue damage in rats
- 15 January 2008
- journal article
- Published by Wiley in Journal of Pineal Research
- Vol. 44 (4), 432-438
- https://doi.org/10.1111/j.1600-079x.2007.00546.x
Abstract
Endosulfan is a chlorinated cyclodiene insecticide which induces oxidative stress. In this study, we investigated the possible protective effect of melatonin, an antioxidant agent, against endosulfan (Endo)-induced toxicity in rats. Wistar albino rats (n = 8) were administered endosulfan (22 mg/kg/day orally) followed by either saline (Endo group) or melatonin (10 mg/kg/day, Endo + Mel group) for 5 days. In other rats, saline (control group) or melatonin (10 mg/kg/day, Mel group) was injected for 5 days, following corn oil administration (vehicle of endosulfan). Measurement of malondialdehyde (MDA) and glutathione (GSH) levels, myeloperoxidase (MPO) activity and collagen content were performed in liver and kidney. Furthermore, aspartate aminotransferase (AST), alanine aminotransferase (ALT), blood urea nitrogen (BUN), and creatinine levels, lactate dehydrogenase (LDH) activity were measured in the serum samples, while tumor necrosis factor-alpha (TNF-alpha), interleukin-beta (IL-beta) and total antioxidant capacity (AOC) were assayed in plasma samples. Endosulfan administration caused a significant decrease in tissue GSH and plasma AOC, which was accompanied with significant rises in tissue MDA and collagen levels and MPO activity. Moreover, the proinflammatory mediators (TNF-alpha and IL-beta), LDH activity, AST, ALT, creatinine and BUN levels were significantly elevated in the endosulfan-treated rats. On the other hand, melatonin treatment reversed all these biochemical alterations induced by endosulfan. Our results suggest that oxidative mechanisms play an important role in endosulfan-induced tissue damage and melatonin, by inhibiting neutrophil infiltration, balancing oxidant-antioxidant status and regulating the generation of inflammatory mediators, ameliorates oxidative organ injury as a result of endosulfan toxicity.Keywords
This publication has 37 references indexed in Scilit:
- One molecule, many derivatives: A never‐ending interaction of melatonin with reactive oxygen and nitrogen species?Journal of Pineal Research, 2006
- Interactions of melatonin and its metabolites with the ABTS cation radical: extension of the radical scavenger cascade and formation of a novel class of oxidation products, C2‐substituted 3‐indolinonesJournal of Pineal Research, 2006
- Protective effect of aqueous garlic extract against naphthalene-induced oxidative stress in miceJournal of Pharmacy and Pharmacology, 2005
- Visualization of the antioxidative effects of melatonin at the mitochondrial level during oxidative stress‐induced apoptosis of rat brain astrocytesJournal of Pineal Research, 2004
- Melatonin is a scavenger of peroxynitriteLife Sciences, 1997
- Melatonin: A peroxyl radical scavenger more effective than vitamin ELife Sciences, 1994
- Assessment of myeloperoxidase activity in whole rat kidneyJournal of Pharmacological Methods, 1990
- Glutathione, free radicals and chemotherapeutic agents: Mechanisms of free-radical induced toxicity and glutathione-dependent protectionPharmacology & Therapeutics, 1988
- A simple micromethod for collagen and total protein determination in formalin-fixed paraffin-embedded sections.Journal of Histochemistry & Cytochemistry, 1985
- Plasma Creatinine Determination A New and Specific Jaffe Reaction MethodScandinavian Journal of Clinical and Laboratory Investigation, 1965