Staphylococcus aureus Virulence Expression Is Impaired by Lactococcus lactis in Mixed Cultures
- 1 July 2009
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 75 (13), 4459-4472
- https://doi.org/10.1128/aem.02388-08
Abstract
Staphylococcus aureus is responsible for numerous food poisonings due to the production of enterotoxins by strains contaminating foodstuffs, especially dairy products. Several parameters, including interaction with antagonistic flora such as Lactococcus lactis , a lactic acid bacterium widely used in the dairy industry, can modulate S. aureus proliferation and virulence expression. We developed a dedicated S. aureus microarray to investigate the effect of L. lactis on staphylococcal gene expression in mixed cultures. This microarray was used to establish the transcriptomic profile of S. aureus in mixed cultures with L. lactis in a chemically defined medium held at a constant pH (6.6). Under these conditions, L. lactis hardly affected S. aureus growth. The expression of most genes involved in the cellular machinery, carbohydrate and nitrogen metabolism, and stress responses was only slightly modulated: a short time lag in mixed compared to pure cultures was observed. Interestingly, the induction of several virulence factors and regulators, including the agr locus, sarA , and some enterotoxins, was strongly affected. This work clearly underlines the complexity of L. lactis antagonistic potential for S. aureus and yields promising leads for investigations into nonantibiotic biocontrol of this major pathogen.Keywords
This publication has 95 references indexed in Scilit:
- Transcriptomic Response of Lactococcus lactis in Mixed Culture with Staphylococcus aureusApplied and Environmental Microbiology, 2009
- RNAIII-Independent Target Gene Control by the agr Quorum-Sensing System: Insight into the Evolution of Virulence Regulation in Staphylococcus aureusMolecular Cell, 2008
- A global view of Staphylococcus aureus whole genome expression upon internalization in human epithelial cellsBMC Genomics, 2007
- Influence of the Two-Component System SaeRS on Global Gene Expression in Two Different Staphylococcus aureus StrainsJournal of Bacteriology, 2006
- Global Regulatory Impact of ClpP Protease ofStaphylococcus aureuson Regulons Involved in Virulence, Oxidative Stress Response, Autolysis, and DNA RepairJournal of Bacteriology, 2006
- Antibacterial activity of lactic acid bacteria against spoilage and pathogenic bacteria isolated from the same meat small-scale facility: 2—Behaviour of pathogenic and spoilage bacteria in dual species biofilms including a bacteriocin-like-producing lactic acid bacteriaFood Control, 2006
- Understanding the rise of the superbug: investigation of the evolution and genomic variation of Staphylococcus aureusFunctional & Integrative Genomics, 2006
- RNAIII inhibiting peptide (RIP) inhibits agr-regulated toxin productionPeptides, 2001
- Exfoliatin-Producing Strains Define a Fourth agr Specificity Group in Staphylococcus aureusJournal of Bacteriology, 2000
- Regulation of exoprotein gene expression in Staphylococcus aureus by agrMolecular Genetics and Genomics, 1986