CHOLINESTERASE IN DENERVATED END PLATES AND MUSCLE FIBRES
Open Access
- 1 November 1963
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 19 (2), 349-358
- https://doi.org/10.1083/jcb.19.2.349
Abstract
Parallel studies were made of cholinesterase activities and localizations in denervated rat and rabbit gastrocnemius muscle. Koelle's histochemical reaction was used for demonstrating the localization of cholinesterases. Enzyme activities in whole sliced muscle were measured by electrometric titration. The Cartesian ampulla-diver technique was used for cholinesterase activity determinations in end plate regions or in small pieces of the muscle fibre itself. No changes in the activity of cholinesterases (ChE) were found in the whole denervated muscle which would account for its chemical supersensitivity. The ChE distribution pattern was changed so that the end plate region became less active in the denervated muscle than in the normal one. The decrease in ChE activity in the end plates seems to be largely compensated for by an increase of this enzyme elsewhere in the muscle. A possible connection between the spatial spread of cholinesterase activity and the enlargement of the acetylcholine-sensitive surface is discussed.Keywords
This publication has 21 references indexed in Scilit:
- Investigations on the physiological role of myosincholinesterase in phylogenesisJournal of Cellular and Comparative Physiology, 1961
- [Relations between cholinergic receptors and acetylcholinesterase of the end-plates, examined during the degeneration and regeneration of the mouse diaphragm].1961
- Effects of Motor Innervation on the Chemical Sensitivity of Skeletal MusclePhysiological Reviews, 1960
- The acetylcholine sensitivity of frog muscle fibres after complete or partial devervation.1960
- A study of supersensitivity in denervated mammalian skeletal muscleThe Journal of Physiology, 1959
- [Radioautography of end-plates with radiocarbon-labeled curarine after denervation and administration of prostigmine and SKF-525A].1958
- Some Histochemical Observations on Human Dystrophic MuscleScience, 1957
- Kinetik der Cholinesterase und Probleme der Erregungsübertrngung und auslösung1Acta Physiologica Scandinavica, 1953
- Cholinesterase content of normal and denervated skeletal muscle in the guinea‐pigThe Journal of Physiology, 1952
- On the contraction of muscle, chiefly in relation to the presence of ‘receptive’ substancesThe Journal of Physiology, 1907