A canine ex vivo shunt for isotopic hemocompatibility evaluation of a NHLBI DTB primary reference material and of a IUPAC reference material
- 1 January 1994
- journal article
- Published by Informa UK Limited in Journal of Biomaterials Science, Polymer Edition
- Vol. 5 (4), 279-291
- https://doi.org/10.1163/156856294x00022
Abstract
Factors determining the thrombogenic response to particular artificial surfaces were investigated ex vivo in a canine shunt model. Methods using radioisotopic tracers made it possible to dynamically monitor the deposition of labelled blood cells and proteins on a NHLBI.DTB primary reference material polydimethylsiloxane (PRM.PDMS) and on a IUPAC reference material polyvinyl chloride (IUPAC.PVC). On the one hand, leukocyte affinity τs(leu) (number of deposited leukocytes mm-2 S-1) was not significantly different between IUPAC.PVC (τs(leu) = 1.2-2.5) and PRM.PDMS (τs(leu) = 1.5-3.4) and the fibrinogen adsorption rate varied from 33 to 48.10-5 μg mm-2 s-1 for both these materials. On the other hand, platelet affinity τs(plat) (number of deposited platelets mm-2 s-1) was significantly different (p < 0.05) for IUPAC.PVC and PRM.PDMS (τs(plat)PVC = 683 ± 200 > τs(plat)PDMS - 327 ± 80). Scanning electron micrographs of adherent platelets, red cells and leukocytes after blood contact ex vivo were performed after each experiment. This preliminary work contributes not only to quantify the adsorption of different radiotracers, but also to evaluate the superficial distribution of the labelled biological species on the inner surface of the tested biomaterials.Keywords
This publication has 12 references indexed in Scilit:
- Acute and chronic canine ex vivo blood interactions with NHLBI-DTB Primary Reference MaterialsBiomaterials, 1989
- Experimental evaluation of a gelatin-coated polyester graft used as an arterial sstituteBiomaterials, 1989
- In vivo leucocyte interactions with the NHLBI-DTB primary reference materials: Polyethylene and silica-free polydimethylsiloxaneBiomaterials, 1987
- Topographic and Dynamic Radioisotopic Investigations of Interactions Between Biomaterials and Blood ComponentsArtificial Organs, 1986
- Soluble fibrinogen derivatives generated by thrombin:Affinity for elastikThrombosis Research, 1986
- In-111 labeled leukocytes: a review of problems in image interpretation.Radiology, 1985
- A canineex vivo series shunt for evaluating thrombus deposition on polymer surfacesJournal of Biomedical Materials Research, 1984
- Direct in vivo study of flowing blood-artificial surface interactions — An original application of dynamic isotopic techniquesThe International Journal of Applied Radiation and Isotopes, 1980
- Indium-111 labeled platelets: Studies on preparation and evaluation of in vitro and in vivo functionsThrombosis Research, 1976
- A new stannous agent kit for labelling red blood cells with99Tcmand its clinical applicationThe British Journal of Radiology, 1976