Cross-Reactivity of Herpesvirus-Specific CD8 T Cell Lines Toward Allogeneic Class I MHC Molecules
Open Access
- 12 August 2010
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 5 (8), e12120
- https://doi.org/10.1371/journal.pone.0012120
Abstract
Although association between persistent viral infection and allograft rejection is well characterized, few examples of T-cell cross-reactivity between self-MHC/viral and allogeneic HLA molecules have been documented so far. We appraised in this study the alloreactivity of CD8 T cell lines specific for immunodominant epitopes from human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV). CD8 T cell lines were generated after sorting with immunomagnetic beads coated with either pp65495–503/A*0201, BMLF1259–267/A*0201, or BZLF154–64/B*3501 multimeric complexes. Alloreactivity of the CD8 T cell lines against allogeneic class I MHC alleles was assessed by screening of (i) TNF-α production against COS-7 cells transfected with as many as 39 individual HLA class I-encoding cDNA, and (ii) cytotoxicity activity toward a large panel of HLA-typed EBV-transformed B lymphoblastoid cell lines. We identified several cross-reactive pp65/A*0201-specific T cell lines toward allogeneic HLA-A*3001, A*3101, or A*3201. Moreover, we described here cross-recognition of HLA-Cw*0602 by BZLF1/B*3501-specific T cells. It is noteworthy that these alloreactive CD8 T cell lines showed efficient recognition of endothelial cells expressing the relevant HLA class I allele, with high level TNF-α production and cytotoxicity activity. Taken together, our data support the notion that herpes virus-specific T cells recognizing allo-HLA alleles may promote solid organ rejection.Keywords
This publication has 51 references indexed in Scilit:
- Allo-HLA reactivity of virus-specific memory T cells is commonBlood, 2010
- Acceleration of allograft failure by cytomegalovirusCurrent Opinion in Immunology, 2007
- Impact of CD8–MHC class I interaction in detection and sorting efficiencies of antigen-specific T cells using MHC class I/peptide multimers: contribution of pMHC valencyInternational Immunology, 2006
- Allogeneic MHC Class I Molecules With Numerous Sequence Differences Do Not Elicit a CTL ResponseHuman Immunology, 2005
- Broadly targeted human cytomegalovirus-specific CD4+ and CD8+ T cells dominate the memory compartments of exposed subjectsThe Journal of Experimental Medicine, 2005
- Non-HLA-Type Endothelial Cell Reactive Alloantibodies in Pre-Transplant Sera of Kidney Recipients Trigger ApoptosisAmerican Journal of Transplantation, 2003
- Cross‐reactive memory T cells for Epstein‐Barr virus augment the alloresponse to common human leukocyte antigens: degenerate recognition of major histocompatibility complex‐bound peptide by T cells and its role in alloreactivityEuropean Journal of Immunology, 1997
- T‐Cell Receptor Usage of Major Histocompatibility Complex Class I Restricted Peptide‐Specific T‐LymphocytesAnnals of the New York Academy of Sciences, 1995
- An alloresponse in humans is dominated by cytotoxic T lymphocytes (CTL) cross-reactive with a single Epstein-Barr virus CTL epitope: implications for graft-versus-host disease.The Journal of Experimental Medicine, 1994