Coffee Leaf Scorch Caused by a Strain of Xylella fastidiosa from Citrus
Open Access
- 1 May 2001
- journal article
- Published by Scientific Societies in Plant Disease
- Vol. 85 (5), 501-505
- https://doi.org/10.1094/pdis.2001.85.5.501
Abstract
Citrus variegated chlorosis (CVC) and coffee leaf scorch (CLS) are two economically important diseases in Brazil caused by the bacterium Xylella fastidiosa. Strains of the bacterium isolated from the two plant hosts are very closely related, and the two diseases share sharpshooter insect vectors. In order to determine if citrus strains of X. fastidiosa could infect coffee and induce CLS disease, plant inoculations were performed. Plants of coffee, Coffea arabica ‘Mundo Novo’, grafted on Coffea canephora var. robusta ‘Apuatão 2258’ were mechanically inoculated with triply cloned strains of X. fastidiosa isolated from diseased coffee and citrus. Three months postinoculation, 5 of the 10 plants inoculated with CLS-X. fastidiosa and 1 of the 10 plants inoculated with CVC-X. fastidiosa gave positive enzyme-linked immunosorbent assay (ELISA) and/or polymerase chain reaction (PCR). Eight months postinoculation, another six plants inoculated with CVC-X. fastidiosa gave positive PCR results. The two X. fastidiosa strains were isolated from the inoculated plants and showed the same characteristics as the original clones by microscopy, ELISA, and PCR. None of the plants inoculated with sterile periwinkle wilt (PW) medium as controls gave positive reactions in diagnostic tests, and none developed disease symptoms. Six months postinoculation, seven plants inoculated with CLS-X. fastidiosa and eight inoculated with CVC-X. fastidiosa began to develop characteristic CLS symptoms, including apical and marginal leaf scorch, defoliation, and reductions of internode length, leaf size, and plant height, terminal clusters of small chlorotic and deformed leaves, and lateral shoot dieback. We have demonstrated that X. fastidiosa from citrus plants is pathogenic for coffee plants. This has important consequences for the management of CLS disease and has implications for the origin of citrus variegated chlorosis disease.Keywords
This publication has 12 references indexed in Scilit:
- Strains of Xylella fastidiosa rapidly distinguished by arbitrarily primed-PCR.Current Microbiology, 2000
- A Triply Cloned Strain of Xylella fastidiosa Multiplies and Induces Symptoms of Citrus Variegated Chlorosis in Sweet OrangeCurrent Microbiology, 1999
- Diversity of a Xylella fastidiosa Population Isolated from Citrus sinensis Affected by Citrus Variegated Chlorosis in BrazilSystematic and Applied Microbiology, 1998
- Coffee Leaf Scorch Bacterium: Axenic Culture, Pathogenicity, and Comparison with Xylella fastidiosa of CitrusPlant Disease, 1998
- Citrus Variegated Chlorosis Bacterium: Axenic Culture, Pathogenicity, and Serological Relationships with Other Strains ofXylella fastidiosaPhytopathology®, 1994
- Culture and serological detection of the xylem-limited bacterium causing citrus variegated chlorosis and its identification as a strain ofXylella fastidiosaCurrent Microbiology, 1993
- Pathogenicity of Xylella fastidiosa in American Elm and Failure of Reciprocal Transmission Between Strains from Elm and SycamorePlant Disease, 1993
- Multiplication and Translocation ofXylella fastidiosain Petioles and Stems of Grapevine Resistant, Tolerant, and Susceptible to Pierce's DiseasePhytopathology®, 1990
- Periwinkle Wilt Bacterium: Axenic Culture, Pathogenicity, and Relationships to Other Gram-Negative, Xylem-Inhabiting BacteriaPhytopathology®, 1983
- Etiology of Almond Leaf Scorch Disease and Transmission of the Causal AgentPhytopathology®, 1976