Transcriptional profiling of in vitro smooth muscle cell differentiation identifies specific patterns of gene and pathway activation
- 17 November 2004
- journal article
- research article
- Published by American Physiological Society in Physiological Genomics
- Vol. 19 (3), 292-302
- https://doi.org/10.1152/physiolgenomics.00148.2004
Abstract
Mesodermal and epidermal precursor cells undergo phenotypic changes during differentiation to the smooth muscle cell (SMC) lineage that are relevant to pathophysiological processes in the adult. Molecular mechanisms that underlie lineage determination and terminal differentiation of this cell type have received much attention, but the genetic program that regulates these processes has not been fully defined. Study of SMC differentiation has been facilitated by development of the P19-derived A404 embryonal cell line, which differentiates toward this lineage in the presence of retinoic acid and allows selection for cells adopting a SMC fate through a differentiation-specific drug marker. We sought to define global alterations in gene expression by studying A404 cells during SMC differentiation with oligonucleotide microarray transcriptional profiling. Using an in situ 60-mer array platform with more than 20,000 mouse genes derived from the National Institute on Aging clone set, we identified 2,739 genes that were significantly upregulated after differentiation was completed (false-detection ratioKeywords
This publication has 45 references indexed in Scilit:
- Ets ternary complex transcription factorsGene, 2003
- Gene expression profiling of resting and activated vascular smooth muscle cells by serial analysis of gene expression and clustering analysisGenomics, 2003
- Serum response factor function and dysfunction in smooth muscleRespiratory Physiology & Neurobiology, 2003
- In Situ-Synthesized Novel Microarray Optimized for Mouse Stem Cell and Early Developmental Expression ProfilingGenome Research, 2003
- Cysteine-Rich LIM-Only Proteins CRP1 and CRP2 Are Potent Smooth Muscle Differentiation CofactorsDevelopmental Cell, 2003
- Composition and Function of AP-1 Transcription Complexes during Muscle Cell DifferentiationJournal of Biological Chemistry, 2002
- Positive- and Negative-acting Krüppel-like Transcription Factors Bind a Transforming Growth Factor β Control Element Required for Expression of the Smooth Muscle Cell Differentiation Marker SM22α in VivoJournal of Biological Chemistry, 2000
- Gene Ontology: tool for the unification of biologyNature Genetics, 2000
- A family of chromatin remodeling factors related to Williams syndrome transcription factorProceedings of the National Academy of Sciences of the United States of America, 2000
- A Retinoic Acid–Induced Clonal Cell Line Derived From Multipotential P19 Embryonal Carcinoma Cells Expresses Smooth Muscle CharacteristicsCirculation Research, 1995