Drak2 overexpression results in increased β‐cell apoptosis after free fatty acid stimulation
- 5 September 2008
- journal article
- research article
- Published by Wiley in Journal of Cellular Biochemistry
- Vol. 105 (4), 1073-1080
- https://doi.org/10.1002/jcb.21910
Abstract
Drak2 is a serine threonine kinase in the death-associated protein family. In this study, we investigated its role in free fatty acid (FFA)-induced islet apoptosis. Drak2 mRNA and protein were rapidly induced in islet β-cells after FFA stimulation. Such Drak2 upregulation was accompanied by increased β-cell apoptosis, which was inhibited by Drak2 knockdown using siRNA. Conversely, transgenic (Tg) Drak2 overexpression led to aggravated β-cell apoptosis triggered by FFA. Drak2 overexpression in islets compromised the increase of anti-apoptotic factors, such as Bcl-2, Bcl-xL and Flip, upon FFA assault. Further in vivo experiments demonstrated that Drak2 Tg mice presented compromised glucose tolerance in a diet-induced obesity model. Our data show that Drak2 is detrimental to islet survival in the presence of excessive lipid. J. Cell. Biochem. 105: 1073–1080, 2008.Keywords
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