Biochemical studies with infectious bursal disease virus: Comparison of some of its properties with infectious pancreatic necrosis virus
- 1 September 1979
- journal article
- research article
- Published by Springer Science and Business Media LLC in Archiv für die gesamte Virusforschung
- Vol. 60 (3-4), 265-277
- https://doi.org/10.1007/bf01317498
Abstract
Infectious bursal disease (IBD) virus was purified from the bursae of infected chickens. Two morphologically indistinguishable populations of virus particles were separated in sucrose gradients and possessed sedimentation coefficients of 395S and 460S. Both populations contained RNA and had identical polypeptide compositions. IBD virus banded at a density of 1.31 g/ml in CsCl and at 1.24 g/ml in sodium potassium tartrate. IBD virus contained two RNA segments with mol. wts. of 2.4×106 and 2.2×106 as estimated by polyacrylamide-agarose gel electrophoresis, but sedimented in sucrose gradients at 15S. Virus RNA was resistant to 0.1 µg/ml ribonuclease treatment under conditions in which ribosomal RNA was completely hydrolysed, but was sensitive to 1.0 and 10 µg/ml treatments. These results suggest that the RNA consists of either double-stranded or highly ordered single-stranded molecules. IBD virus contained seven polypeptides with mol. wts. in the range 97,000 to 24,000. Two polypeptides were absent in empty particles of IBD virus. IBD and infectious pancreatic necrosis (IPN) viruses were morphologically indistinguishable. IPN virus possessed a sedimentation coefficient of 440S and banded at a density of 1.32 g/ml in CsCl. In addition the electrophoretic mobilities of IBD and IPN virus RNAs were almost identical. Polyacrylamide slab gel electrophoresis showed that while the number and size of the polypeptides were different for each virus there were similarities in the overall pattern.Keywords
This publication has 15 references indexed in Scilit:
- BOVINE ENTEROVIRUSESAnnals of the New York Academy of Sciences, 2006
- Cell culture studies with a cytopathic bovine rotavirusArchiv für die gesamte Virusforschung, 1977
- Relationship of a Virus from Tellina tenuis to Infectious Pancreatic Necrosis VirusJournal of General Virology, 1977
- Characterization of Pig Rotavirus RNAJournal of General Virology, 1976
- Neuraminidase Content of Strains of Newcastle Disease Virus which Differ in VirulenceJournal of General Virology, 1975
- Infectious bursal disease agent: Morphology by negative stain electron microscopyArchiv für die gesamte Virusforschung, 1975
- A Possible Precursor containing RNA of a Bovine Enterovirus: the Provirion 11Journal of General Virology, 1974
- Physico-chemical and Morphological Features of Infectious Pancreatic Necrosis VirusJournal of General Virology, 1973
- Antigenically-related Viruses Associated with Infectious Bursal DiseaseJournal of General Virology, 1973
- Antiviral Activity of Double Stranded RNA and Virus-like Particles from Penicillium stoloniferumNature, 1970