Screening of Commercial Hydrolases for the Degradation of Ochratoxin A
- 3 October 2000
- journal article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 48 (11), 5736-5739
- https://doi.org/10.1021/jf000413j
Abstract
Ochratoxin A (OTA) is a nephrotoxic and carcinogenic mycotoxin. The toxin is a common contaminant of various foods and feeds and poses a serious threat to the health of both humans and animals. A number of commercial hydrolases were screened for the ability to degrade OTA to nontoxic compounds. A crude lipase from Aspergillus niger (Amano A) proved to substantially hydrolyze OTA to the nontoxic OTalpha and phenylalanine, as confirmed by HPLC with fluorescence detection. The enzyme was purified by anion exchange chromatography to homogeneity. Activity staining of the purified enzyme with alpha-naphthyl acetate/Fast Red revealed only one band exhibiting hydrolytic activity. The specific activity of the purified enzyme toward OTA was 2.32 units/mg.This publication has 5 references indexed in Scilit:
- Detoxification of mycotoxin-contaminated food and feed by microorganismsTrends in Food Science & Technology, 1999
- Ochratoxin A production by strains of Aspergillus niger var. nigerApplied and Environmental Microbiology, 1994
- A review of recent advances in understanding ochratoxicosisJournal of Animal Science, 1992
- Ochratoxin A in human blood in relation to Balkan endemic nephropathy and urinary system tumours in BulgariaFood Additives & Contaminants, 1988
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976