Three-Dimensional Tracking of Small Aquatic Organisms Using Fluorescent Nanoparticles
Open Access
- 7 November 2013
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 8 (11), e78498
- https://doi.org/10.1371/journal.pone.0078498
Abstract
Tracking techniques are vital for the understanding of the biology and ecology of organisms. While such techniques have provided important information on the movement and migration of large animals, such as mammals and birds, scientific advances in understanding the individual behaviour and interactions of small (mm-scale) organisms have been hampered by constraints, such as the sizes of existing tracking devices, in existing tracking methods. By combining biology, chemistry and physics we here present a method that allows three-dimensional (3D) tracking of individual mm-sized aquatic organisms. The method is based on in-vivo labelling of the organisms with fluorescent nanoparticles, so-called quantum dots, and tracking of the organisms in 3D via the quantum-dot fluorescence using a synchronized multiple camera system. It allows for the efficient and simultaneous study of the behaviour of one as well as multiple individuals in large volumes of observation, thus enabling the study of behavioural interactions at the community scale. The method is non-perturbing – we demonstrate that the labelling is not affecting the behavioural response of the organisms – and is applicable over a wide range of taxa, including cladocerans as well as insects, suggesting that our methodological concept opens up for new research fields on individual behaviour of small animals. Hence, this offers opportunities to focus on important biological, ecological and behavioural questions never before possible to address.This publication has 25 references indexed in Scilit:
- Tracking the Small with the Smallest – Using Nanotechnology in Tracking ZooplanktonPLOS ONE, 2010
- Effects of ultraviolet radiation on pigmentation, photoenzymatic repair, behavior, and community ecology of zooplanktonPhotochemical & Photobiological Sciences, 2009
- Size-structured risk assessments govern Daphnia migrationProceedings. Biological sciences, 2008
- Swimming and escape behavior of copepod nauplii: implications for predator-prey interactions among copepodsMarine Ecology Progress Series, 2001
- Induced pigmentation in zooplankton: a trade–off between threats from predation and ultraviolet radiationProceedings. Biological sciences, 2000
- Quantum Dot Bioconjugates for Ultrasensitive Nonisotopic DetectionScience, 1998
- Mate-finding behaviour in Calanus marshallae FrostPhilosophical Transactions B, 1998
- Observing free-swimming copepods matingPhilosophical Transactions B, 1998
- Locating a mate in 3D: the case of Temora longicornisPhilosophical Transactions B, 1998
- Spatio‐temporal patterns in the diel vertical migration of the copepod Metridia lucens in the northeast Atlantic derived from the Continuous Plankton Recorder surveyLimnology and Oceanography, 1995