Investigation of Doxorubicin Resistance in MCF-7 Breast Cancer Cells Using Shot-Gun Comparative Proteomics with Proteolytic 18O Labeling
- 13 February 2004
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Proteome Research
- Vol. 3 (3), 455-462
- https://doi.org/10.1021/pr0340835
Abstract
A shot-gun comparative proteomic investigation utilizing proteolytic 18O labeling has been carried out on a drug susceptible MCF-7 human breast cancer cell line and a related cell line that is resistant to doxorubicin. The proteolytic 18O labeling method has been further refined and optimized for application to a protein fraction stemming from the cytosol of the breast cancer cells. The comparative investigation revealed several proteins with altered expression levels in the doxorubicin resistant line. These altered proteins are considered for a possible role in doxorubicin resistance. Keywords: proteolytic labeling • drug resistance • breast cancer cellsKeywords
This publication has 39 references indexed in Scilit:
- Apoptosis-related fragmentation, translocation, and properties of human prothymosin alphaExperimental Cell Research, 2003
- Rapid upregulation of telomerase activity in human leukemia HL-60 cells treated with clinical doses of the DNA-damaging drug etoposideLeukemia, 2002
- Integrin signaling inhibits paclitaxel-induced apoptosis in breast cancer cellsOncogene, 2001
- Ca2+-free Calmodulin and Calmodulin Damaged byin Vitro Aging Are Selectively Degraded by 26 S Proteasomes without UbiquitinationPublished by Elsevier BV ,2000
- A critical evaluation of the mechanisms of action proposed for the antitumor effects of the anthracycline antibiotics adriamycin and daunorubicinBiochemical Pharmacology, 1999
- Redox control of resistance to cis-diamminedichloroplatinum (II) (CDDP): protective effect of human thioredoxin against CDDP-induced cytotoxicity.JCI Insight, 1996
- Reversion of the P-glycoprotein-mediated multidrug resistance of cancer cells by FK-506 derivativesAnti-Cancer Drugs, 1993
- Thioredoxin regenerates proteins inactivated by oxidative stress in endothelial cellsEuropean Journal of Biochemistry, 1992
- Mechanisms of Multidrug Resistance in Cancer TreatmentActa Oncologica, 1992
- Possible role for metallothionein in protection against radiation-induced oxidative stress. Kinetics and mechanism of its reaction with superoxide and hydroxyl radicalsBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1985