Effect of increasing ratio of estrogen: Androgen on proliferation of normal human prostate stromal and epithelial cells, and the malignant cell line LNCaP
- 21 August 2005
- journal article
- research article
- Published by Wiley in The Prostate
- Vol. 66 (1), 105-114
- https://doi.org/10.1002/pros.20327
Abstract
BACKGROUND Changes in steroid ratios seen in the aging male are thought to promote prostate disease. The aims of this study were to compare the effects of varied ratios of steroids on growth of normal stromal and epithelial cell isolates, and the prostate cancer cell line, LNCaP. METHODS The effect of altered steroid ratios on cell proliferation of normal stromal (PrSC) and epithelial (PrEC) prostate cells, and the malignant cell line, LNCaP, were assessed. RESULTS Increasing the ratios of both estrogen:dihydrotestosterone (DHT) and DHT:estrogen, stimulated PrSC proliferation, with increasing estrogen:DHT having the greatest effect. LNCaP proliferation was increased significantly by both steroids, but altered ratios had no additional effect. PrEC proliferation was unaffected when cells were grown alone, despite presence of androgen receptors (AR) and estrogen receptors (ER). When grown in co-culture PrEC cell proliferation was significantly increased by treatments. CONCLUSIONS PrSC proliferation is stimulated by an increasing ratio of estrogen:androgen. Proliferation of normal epithelial cells is stimulated as a result of an indirect action of steroids mediated by stromal cells. Malignant prostate cancer cells have an altered response in comparison.Keywords
This publication has 56 references indexed in Scilit:
- Effects and characterization of paracrine factors produced by human prostate stromal cells in bioassays using rat Sertoli cells, LNCaP tumor cells, and cultured prostate epithelial cellsThe Prostate, 2001
- Cell lineage characteristics of human prostatic stromal cells cultured in vitroThe Prostate, 2000
- Androgen-Stimulated Human Prostate Epithelial Growth Mediated by Stromal-Derived Fibroblast Growth Factor-10.Endocrine Journal, 1999
- Human prostatic smooth muscle cells in culture: Estradiol enhances expression of smooth muscle cell-specific markersThe Prostate, 1997
- Cloning of a novel receptor expressed in rat prostate and ovary.Proceedings of the National Academy of Sciences of the United States of America, 1996
- Insulin-like growth factor axis abnormalities in prostatic stromal cells from patients with benign prostatic hyperplasiaJournal of Clinical Endocrinology & Metabolism, 1994
- Androgen metabolism in the human prostatic cancer cell line LNCaPThe Journal of Steroid Biochemistry and Molecular Biology, 1994
- The androgen receptor in LNCaP cells contains a mutation in the ligand binding domain which affects steroid binding characteristics and response to antiandrogensThe Journal of Steroid Biochemistry and Molecular Biology, 1992
- A mutation in the ligand binding domain of the androgen receptor of human INCaP cells affects steroid binding characteristics and response to anti-androgensBiochemical and Biophysical Research Communications, 1990
- Regulation of cytoplasmic dihydrotestosterone binding in dog prostate by 17 beta-estradiol.JCI Insight, 1979