Green fluorescent protein: A perspective
Open Access
- 28 June 2011
- journal article
- review article
- Published by Wiley in Protein Science
- Vol. 20 (9), 1509-1519
- https://doi.org/10.1002/pro.684
Abstract
A brief personal perspective is provided for green fluorescent protein (GFP), covering the period 1994–2011. The topics discussed are primarily those in which my research group has made a contribution and include structure and function of the GFP polypeptide, the mechanism of fluorescence emission, excited state protein transfer, the design of ratiometric fluorescent protein biosensors and an overview of the fluorescent proteins derived from coral reef animals. Structure‐function relationships in photoswitchable fluorescent proteins and nonfluorescent chromoproteins are also briefly covered.Keywords
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