Oocyte Cryopreservation Based in Sheep: The Current Status and Future Perspective
- 1 December 2017
- journal article
- review article
- Published by Mary Ann Liebert Inc in Biopreservation and Biobanking
- Vol. 15 (6), 535-547
- https://doi.org/10.1089/bio.2017.0074
Abstract
The establishment of a cryopreservation procedure for sheep oocytes will enhance the development of long-term conservation of sheep female genetic resources and the advancement of sheep embryonic biotechnology. However, higher cytoplasmic lipid content and complex subcellular structures affect the relatively higher sensitivity of oocytes to chilling and freezing stresses. Currently, the reports related to sheep oocyte cryopreservation lag behind bovine or pig studies. A standardized freezing procedure has not been established. The present mainstream viewpoint favors the superiority of vitrification over conventional slow-freezing methods. The combination of permeable and impermeable cryoprotectants plus vitrification at a fast cooling/warming velocity benefits the cryosurvival of sheep oocytes. In this review, the research status and the cryoinjury mechanism of sheep oocyte cryopreservation will be reviewed in detail. Moreover, some technological highlights potentially influencing survival of cryopreserved sheep oocytes, such as delipidation, high hydrostatic pressure, or loading trehalose into cytoplasm, are summarized. Meanwhile, the future perspectives in the field of sheep oocyte cryopreservation will be discussed.Keywords
This publication has 132 references indexed in Scilit:
- Raman microspectroscopy as a non-invasive tool to assess the vitrification-induced changes of ovine oocyte zona pellucidaCryobiology, 2012
- Pathway for the Movement of Water and Cryoprotectants in Bovine Oocytes and Embryos1Biology of Reproduction, 2011
- Survival of mouse oocytes after being cooled in a vitrification solution to −196°C at 95° to 70,000°C/min and warmed at 610° to 118,000°C/min: A new paradigm for cryopreservation by vitrificationCryobiology, 2011
- Current progress in oocyte and embryo cryopreservation by slow freezing and vitrificationReproduction, 2011
- In vitro maturation, apoptotic gene expression and incidence of numerical chromosomal abnormalities following cryotop vitrification of sheep cumulus-oocyte complexesJournal of Assisted Reproduction and Genetics, 2010
- The dominance of warming rate over cooling rate in the survival of mouse oocytes subjected to a vitrification procedureCryobiology, 2009
- Cryopreservation of porcine oocytes: recent advancesMolecular Human Reproduction, 2009
- Animal oocyte and embryo cryopreservationCell and Tissue Banking, 2008
- Freeze-thaw-induced changes of the zona pellucida explains decreased rates of fertilization in frozen-thawed mouse oocytesReproduction, 1990
- Cryopreservation of Drosophila melanogaster embryosNature, 1990