Nuclei from Rat Liver: Isolation Method That Combines Purity with High Yield
- 30 December 1966
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 154 (3757), 1662-1665
- https://doi.org/10.1126/science.154.3757.1662
Abstract
A procedure is described that gives almost quantitative separation of clean nuclei from a homogenate of rat liver. It is a modification of methods that use concentrated sucrose solutions rather than citric acid or detergents and, therefore, permits further quantitative fractionation of cytoplasmic components.This publication has 10 references indexed in Scilit:
- Enzyme levels in rats adapted to 36-hour fastingAdvances in Enzyme Regulation, 1966
- RNA metabolism in the HeLa cell nucleusJournal of Molecular Biology, 1966
- Recent developments in the measurement of nucleic acids in biological materials. A supplementary reviewThe Analyst, 1966
- ISOLATION OF NUCLEI FROM MAMMALIAN TISSUES THROUGH THE USE OF TRITON X-100Journal of Histochemistry & Cytochemistry, 1964
- STUDIES ON ISOLATED NUCLEIThe Journal of cell biology, 1963
- The precision of ultraviolet absorption measurements in the Schmidt-Thannhauser procedure for nucleic acid estimationBiochimica et Biophysica Acta, 1962
- Isolation of pure and unaltered liver nuclei morphology and biochemical compositionExperimental Cell Research, 1956
- An ultramicro technique for the determination of deoxypentose nucleic acidArchives of Biochemistry and Biophysics, 1956
- A MICROCHEMICAL DETERMINATION OF DESOXYRIBONUCLEIC ACIDOnline Journal of Public Health Informatics, 1952
- INTRACELLULAR DISTRIBUTION OF ENZYMESOnline Journal of Public Health Informatics, 1948