Cellular dna content parameters in untreated and recurrent squamous cell cancers of the head and neck
Open Access
- 1 May 1989
- Vol. 10 (3), 334-338
- https://doi.org/10.1002/cyto.990100313
Abstract
The presence and degree of DNA aneuploidy as measured by the DNA index (DI) and the S phase fraction (SPF) were determined by flow cytometry in 294 specimens from 237 patients with untreated and recurrent squamous cell carcinomas of the head and neck (SCCHN). A descriptive analysis was performed in which the specimen DNA parameters were correlated with stage, size of primary, degree of lymph node involvement, morphological grade, and treatment status of the corresponding patients. Approximately 70% of the previously untreated specimens contained DNA aneuploid populations (DI > 1.10) and three quarters had SPF that were above 15%. There was a strong, direct as sociation between DI and SPF (P < 0.001). There was no correlation of the presence or degree of DNA aneuploidy with the stage of the tumor or the size of the primary or conventional morphological grade of the tumor. Specimens from patients with recurrent tumors and untreated patients with N3 lymph nodes had significantly lower rates of DNA aneuploidy and mean DI. Serial determinations of DNA aneuploidy in patients with SCCHN undergoing cytotoxic therapy are ongoing and may prove useful in the identification and understanding of resistance and response in this tumor.Keywords
This publication has 17 references indexed in Scilit:
- Solid tumor preparation for clinical application of flow cytometryCytometry, 1987
- Solid tumor preparation for flow cytometry using a standard murine modelCytometry, 1987
- Significance of ploidy in laryngeal cancerThe American Journal of Surgery, 1986
- Flow cytometric analysis of DNA content as a prognostic indicator in squamous cell carcinoma of the tongueThe American Journal of Surgery, 1986
- Flow cytometric analysis of head and neck carcinoma DNA index and S‐fraction from paraffin‐embedded sections: Comparison with malignancy gradingCytometry, 1985
- The analysis and interpretation of DNA distributions measured by flow cytometryCytometry, 1982
- A model for the computer analysis of synchronous DNA distributions obtained by flow cytometryCytometry, 1980
- Multiple Comparisons Using Rank SumsTechnometrics, 1964
- Use of Ranks in One-Criterion Variance AnalysisJournal of the American Statistical Association, 1952