Quantitative analysis of neuronal morphologies in the mouse retina visualized by using a genetically directed reporter
Top Cited Papers
- 22 November 2004
- journal article
- research article
- Published by Wiley in Journal of Comparative Neurology
- Vol. 480 (4), 331-351
- https://doi.org/10.1002/cne.20304
Abstract
An alkaline phosphatase (AP) reporter has been used to visualize detailed morphologies for all major classes of retinal neurons in the adult mouse. The analysis was performed on retinas in which AP expression was activated by Cre‐mediated DNA recombination in a small fraction of cells. Recombination was controlled pharmacologically and, to a first approximation, appears to have occurred randomly. The morphologies of 794 inner retinal neurons have been analyzed by measuring arbor area, stratification level, and neurite branching patterns. When analyzed in this multidimensional parametric space, the cells can be clustered into subgroups by visual inspection and by using the Ward's and K‐means algorithms. One application of this cell morphology data set and cluster analysis is as a standard for comparison with the retinas of genetically altered mice. This work illustrates the utility and feasibility of genetically directed marking methods for large‐scale surveys of neuronal morphology. J. Comp. Neurol. 480:331–351, 2004.Keywords
This publication has 55 references indexed in Scilit:
- The population of bipolar cells in the rabbit retinaJournal of Comparative Neurology, 2004
- Types of bipolar cells in the mouse retinaJournal of Comparative Neurology, 2003
- Large‐scale morphological survey of mouse retinal ganglion cellsJournal of Comparative Neurology, 2002
- The fundamental plan of the retinaNature Neuroscience, 2001
- Unique topographic separation of two spectral classes of cones in the mouse retinaJournal of Comparative Neurology, 1992
- Parasol and midget ganglion cells of the primate retinaJournal of Comparative Neurology, 1989
- Morphological Identification of Serotonin-Accumulating Neurons in the Living RetinaScience, 1986
- Morphological types of horizontal cell in the retina of the domestic catProceedings of the Royal Society of London. B. Biological Sciences, 1978
- Interplexiform cells in rabbit retinaProceedings of the Royal Society of London. B. Biological Sciences, 1977
- Interplexiform cells of the mammalian retina and their comparison with catecholamine-containing retinal cellsProceedings of the Royal Society of London. B. Biological Sciences, 1975