Methodologies for the characterization of microbes in industrial environments: a review
- 1 June 2003
- journal article
- review article
- Published by Oxford University Press (OUP) in Journal of Industrial Microbiology & Biotechnology
- Vol. 30 (6), 327-356
- https://doi.org/10.1007/s10295-003-0056-y
Abstract
There is growing interest in research and development to develop novel tools to study, detect, and characterize microbes and their communities in industrial environments. However, knowledge about their validity in practical industrial use is still scarce. This review describes the advantages and limitations of traditional and molecular methods used for biofilm and/or planktonic cell studies, especially those performed with Listeria monocytogenes, Bacillus cereus, and/or Clostridium perfringens. In addition, the review addresses the importance of isolating the microorganisms from the industrial environment and the possibilities and future prospects for exploiting the described methods in the industrial environment.Keywords
This publication has 64 references indexed in Scilit:
- Quantitative PCRMolecular Biotechnology, 1995
- AFLP: a new technique for DNA fingerprintingNucleic Acids Research, 1995
- Comparison of the fluorescent redox dye 5‐cyano‐2,3‐ditolyltetrazolium chloride with p‐iodonitrotetrazolium violet to detect metabolic activity in heat‐stressed Listeria monocytogenes cellsJournal of Applied Bacteriology, 1994
- A 16S rDNA-based PCR method for rapid and specific detection of Clostridium perfringens in foodMolecular and Cellular Probes, 1994
- A combined PCR and selective enrichment method for rapid detection of Listeria monocytogenesJournal of Applied Bacteriology, 1992
- Detection and identification of Listeria monocytogenes in cooked sausage products and in milk by in vitro amplification of haemolysin gene fragmentsJournal of Applied Bacteriology, 1991
- A medium for the isolation, enumeration and rapid presumptive identification of injuredClostridium perfringensandBacillus cereusJournal of Applied Bacteriology, 1990
- Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms.Proceedings of the National Academy of Sciences of the United States of America, 1989
- The use of direct epifluorescent microscopy (DEM) and the direct epifluorescent filter technique (DEFT) to assess microbial populations on food contact surfacesJournal of Applied Bacteriology, 1988
- Alterations in DNA helix stability due to base modifications can be evaluated using denaturing gradient gel electrophoresisJournal of Molecular Biology, 1987