Peroxidases from Phaeophyceae. I: Extraction and Detection of the Peroxidases

Abstract

Using a newly developed method of extracting peroxidases from Phaeophyceae, samples of 33 different species originating from the Atlantic coast in Brittany [France] were examined. On the basis of the hydrogen peroxide-dependent oxidation of iodide, considerable differences were observed in the amount of peroxidase activity in the various extracts. Season and habitat were shown to have an effect on the results. Peroxidase activity in the crude extracts was also detectable with other typical peroxidase substrates, such as o-dianisidine. These reactions were iodide-dependent. The effect of the pH on peroxidase activity in Ascophyllum nodosum was examined by observing the oxidation of iodide and the iodide-dependent oxidation of o-dianisidine. Differences arising when utilizing both methods of detection can be attributed to non enzymatic secondary reactions.