Clostridium perfringens Enterotoxin Fragment Removes Specific Claudins from Tight Junction Strands
Open Access
- 4 October 1999
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 147 (1), 195-204
- https://doi.org/10.1083/jcb.147.1.195
Abstract
Claudins, comprising a multigene family, constitute tight junction (TJ) strands. Clostridium perfringens enterotoxin (CPE), a single ∼35-kD polypeptide, was reported to specifically bind to claudin-3/RVP1 and claudin-4/CPE-R at its COOH-terminal half. We examined the effects of the COOH-terminal half fragment of CPE (C-CPE) on TJs in L transfectants expressing claudin-1 to -4 (C1L to C4L, respectively), and in MDCK I cells expressing claudin-1 and -4. C-CPE bound to claudin-3 and -4 with high affinity, but not to claudin-1 or -2. In the presence of C-CPE, reconstituted TJ strands in C3L cells gradually disintegrated and disappeared from their cell surface. In MDCK I cells incubated with C-CPE, claudin-4 was selectively removed from TJs with its concomitant degradation. At 4 h after incubation with C-CPE, TJ strands were disintegrated, and the number of TJ strands and the complexity of their network were markedly decreased. In good agreement with the time course of these morphological changes, the TJ barrier (TER and paracellular flux) of MDCK I cells was downregulated by C-CPE in a dose-dependent manner. These findings provided evidence for the direct involvement of claudins in the barrier functions of TJs.Keywords
This publication has 54 references indexed in Scilit:
- Occludin and claudins in tight-junction strands: leading or supporting players?Trends in Cell Biology, 1999
- Functional dissociation of paracellular permeability and transepithelial electrical resistance and disruption of the apical-basolateral intramembrane diffusion barrier by expression of a mutant tight junction membrane protein.The Journal of cell biology, 1996
- Interspecies diversity of the occludin sequence: cDNA cloning of human, mouse, dog, and rat-kangaroo homologues.The Journal of cell biology, 1996
- Occludin: a novel integral membrane protein localizing at tight junctions.The Journal of cell biology, 1993
- Breaking through the tight junction barrier.The Journal of cell biology, 1993
- Tight junction structure and ZO-1 content are identical in two strains of Madin-Darby canine kidney cells which differ in transepithelial resistance.The Journal of cell biology, 1988
- Protective effects of osmotic stabilizers on morphological and permeability alterations induced in vero cells by Clostridium perfringens enterotoxinBiochimica et Biophysica Acta (BBA) - Biomembranes, 1981
- Calcium-independent and dependent steps in action of Clostridiumperfringens enterotoxin on HeLa and Vero cellsBiochemical and Biophysical Research Communications, 1979
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- JUNCTIONAL COMPLEXES IN VARIOUS EPITHELIAThe Journal of cell biology, 1963