Distribution of glutamine synthetase in the snapper (Pagrus auratus) and implications for the immune system
- 1 June 1996
- journal article
- research article
- Published by Springer Science and Business Media LLC in Fish Physiology and Biochemistry
- Vol. 15 (3), 187-194
- https://doi.org/10.1007/bf01875569
Abstract
A sensitive radiotracer method was used to detect glutamine synthetase activity in fish. The distribution of glutamine synthetase activity using this method was shown to be widespread in organs and tissues of the marine finfish, snapper,Pagrus auratus. The optimum pH and temperature for glutamine synthetase activity and Km of glutamate from the brain of the snapper are also reported. Interestingly no glutamine synthetase activity was detected in lymphoid organs, indicating, that as in the mammal, the teleostean immune system is likely to rely on glutamine in circulation for energy and nucleotide synthesis. Furthermore circulating glutamine levels fell as fish became stressed or became ill. Implications of these observations on the function of the immune system of fish are discussed.Keywords
This publication has 49 references indexed in Scilit:
- Nutrition information and training: networking in international community nutritionProceedings of the Nutrition Society, 1992
- The effect of glutamine on murine splenic leukocyte responses to T and B cell mitogensImmunology & Cell Biology, 1990
- Properties of Glutamine Release From Muscle and Its Importance for the Immune SystemJournal of Parenteral and Enteral Nutrition, 1990
- The role of high rates of glycolysis and glutamine utilization in rapidly dividing cellsBioscience Reports, 1985
- Metabolic disorders in severe abdominal sepsis: Glutamine deficiency in skeletal muscleClinical Nutrition, 1982
- Ammonia partitioning between glutamine and urea: Interorgan participation in metabolic acidosisKidney International, 1981
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Glutamine production by the isolated perfused rat heart during ammonium chloride perfusionCardiovascular Research, 1975
- Mechanism of inhibition of glutamine synthetase by methionine sulfoximineBiochemistry, 1969