Active multilayered capsules for in vivo bone formation
- 16 February 2010
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 107 (8), 3406-3411
- https://doi.org/10.1073/pnas.0908531107
Abstract
Interest in the development of new sources of transplantable materials for the treatment of injury or disease has led to the convergence of tissue engineering with stem cell technology. Bone and joint disorders are expected to benefit from this new technology because of the low self-regenerating capacity of bone matrix secreting cells. Herein, the differentiation of stem cells to bone cells using active multilayered capsules is presented. The capsules are composed of poly-L-glutamic acid and poly-L-lysine with active growth factors embedded into the multilayered film. The bone induction from these active capsules incubated with embryonic stem cells was demonstrated in vitro. Herein, we report the unique demonstration of a multilayered capsule-based delivery system for inducing bone formation in vivo. This strategy is an alternative approach for in vivo bone formation. Strategies using simple chemistry to control complex biological processes would be particularly powerful, as they make production of therapeutic materials simpler and more easily controlled.Keywords
This publication has 46 references indexed in Scilit:
- Endochondral bone tissue engineering using embryonic stem cellsProceedings of the National Academy of Sciences of the United States of America, 2008
- Bone morphogenetic proteins in tissue engineering: the road from laboratory to clinic, part II (BMP delivery)Journal of Tissue Engineering and Regenerative Medicine, 2008
- Multiple and time-scheduled in situ DNA delivery mediated by β-cyclodextrin embedded in a polyelectrolyte multilayerProceedings of the National Academy of Sciences of the United States of America, 2006
- Layers of opportunity: nanostructured polymer assemblies for the delivery of macromolecular therapeuticsSoft Matter, 2006
- Engineering Virus Functionalities on Colloidal Polyelectrolyte Lipid CompositesAngewandte Chemie-International Edition, 2005
- Short-Time Tuning of the Biological Activity of Functionalized Polyelectrolyte MultilayersAdvanced Functional Materials, 2005
- Build‐up of Polypeptide Multilayer Coatings with Anti‐Inflammatory Properties Based on the Embedding of Piroxicam–Cyclodextrin ComplexesAdvanced Functional Materials, 2004
- Bioactive Coatings Based on a Polyelectrolyte Multilayer Architecture Functionalized by Embedded ProteinsAdvanced Materials, 2003
- Differentiation plasticity of chondrocytes derived from mouse embryonic stem cellsJournal of Cell Science, 2002
- Novel Hollow Polymer Shells by Colloid-Templated Assembly of PolyelectrolytesAngewandte Chemie-International Edition, 1998