Desulfonauticus submarinus gen. nov., sp. nov., a novel sulfate-reducing bacterium isolated from a deep-sea hydrothermal vent

Abstract

A novel moderately thermophilic, hydrogenotrophic, sulfate-reducing bacterium, strain 6N(T) (=DSM 15269(T)=CIP 107713(T)), was isolated from matrixes of Alvinella and Riftia originating from deep-sea hydrothermal-vent samples collected on the 13 degrees N East-Pacific Rise at a depth of approximately 2600 m. It was a Gram-negative, non-sporulating, curved rod, motile with one polar flagellum, that did not possess desulfoviridin. It grew at temperatures ranging from 30 to 60 degrees C, with an optimum at 45 degrees C, in the presence of 0-5 % NaCl (optimum 2 %). Strain 6N(T) utilized only H(2)/CO(2) and formate as electron donors with acetate as carbon source. Sulfate, sulfite, thiosulfate and elemental sulfur were used as terminal electron acceptors during hydrogen oxidation. The G+C content of DNA was 34.4 mol%. Strain 6N(T) grouped with members of the family Desulfohalobiaceae in the delta-subclass of the PROTEOBACTERIA: Its closest phylogenetic relative was Desulfonatronovibrio hydrogenovorans, with only 90 % similarity between the sequences of the genes encoding 16S rRNA. Because of significant phylogenetic differences from all sulfate-reducing bacteria described so far in the domain Bacteria, this novel thermophile is proposed to be assigned to a new genus and species, Desulfonauticus submarinus gen. nov., sp. nov.