The FHA domain proteins DAWDLE in Arabidopsis and SNIP1 in humans act in small RNA biogenesis
Open Access
- 22 July 2008
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 105 (29), 10073-10078
- https://doi.org/10.1073/pnas.0804218105
Abstract
Proteins containing the forkhead-associated domain (FHA) are known to act in biological processes such as DNA damage repair, protein degradation, and signal transduction. Here we report that DAWDLE (DDL), an FHA domain-containing protein in Arabidopsis, acts in the biogenesis of miRNAs and endogenous siRNAs. Unlike mutants of genes known to participate in the processing of miRNA precursors, such as dcl1, hyponastic leaves1, and serrate, ddl mutants show reduced levels of pri-miRNAs as well as mature miRNAs. Promoter activity of MIR genes, however, is not affected by ddl mutations. DDL is an RNA binding protein and is able to interact with DCL1. In addition, we found that SNIP1, the human homolog of DDL, is involved in miRNA biogenesis and interacts with Drosha. Therefore, we uncovered an evolutionarily conserved factor in miRNA biogenesis. We propose that DDL participates in miRNA biogenesis by facilitating DCL1 to access or recognize pri-miRNAs.Keywords
This publication has 34 references indexed in Scilit:
- Identification of Nuclear Dicing Bodies Containing Proteins for MicroRNA Biogenesis in Living Arabidopsis PlantsCurrent Biology, 2007
- Arabidopsis primary microRNA processing proteins HYL1 and DCL1 define a nuclear body distinct from the Cajal bodyProceedings of the National Academy of Sciences of the United States of America, 2007
- Differential binding of calmodulin-related proteins to their targets revealed through high-density Arabidopsis protein microarraysProceedings of the National Academy of Sciences of the United States of America, 2007
- A diverse and evolutionarily fluid set of microRNAs in Arabidopsis thalianaGenes & Development, 2006
- SERRATE is a novel nuclear regulator in primary microRNA processing in ArabidopsisThe Plant Journal, 2006
- SERRATE: a new player on the plant microRNA sceneEMBO Reports, 2006
- DAWDLE, a Forkhead-Associated Domain Gene, Regulates Multiple Aspects of Plant DevelopmentPlant Physiology, 2006
- The interaction between DCL1 and HYL1 is important for efficient and precise processing of pri-miRNA in plant microRNA biogenesisRNA, 2006
- Expression of Arabidopsis MIRNA GenesPlant Physiology, 2005
- Methylation as a Crucial Step in Plant microRNA BiogenesisScience, 2005