Connective Tissue Progenitors: Practical Concepts for Clinical Applications
- 1 February 2002
- journal article
- review article
- Published by Ovid Technologies (Wolters Kluwer Health)
- Vol. 395 (395), 66-80
- https://doi.org/10.1097/00003086-200202000-00008
Abstract
Tissue engineering can be defined as any effort to create or induce the formation of a specific tissue in a specific location through the selection and manipulation of cells, matrices, and biologic stimuli. The biologic concepts and the biochemical and biophysical principles on which these efforts are based have become an exciting and rapidly evolving field of biomedical research. More importantly, tissue engineering is becoming a clinical reality in the practice of orthopaedic surgery, providing patients and physicians with an expanding set of practical tools for effective therapy. New and improved matrices and bioactive factors inevitably will play important roles in the evolution of orthopaedic tissue engineering. However, tissue engineering never can stray far from fundamental biologic principles, and one of these is that cells do all the work. No new tissue forms except through the activity of living cells. No bone graft, no matrix, no growth factor, no cytokine can contribute to the generation or integration of new tissue, except through the influence it has on the behavior of cells. The efficacy of all current clinical tools depends entirely on the cells in the grafted site, particularly the small subset of stem cells and progenitor cells that are capable of generating new tissue. The current authors review a series of key biologic concepts related to the rational design and selection of composites of cells and matrices in contemporary bone grafting and tissue engineering efforts. The functional paradigms of stem cell biology are reviewed, including self renewal, asymmetric and symmetric mitosis, and lineage restriction. Several potential sources for autogenous stem cells for connective tissues are discussed. Finally, a simple mathematical model is introduced as a tool for understanding the functional demands placed on stem cells and progenitors in a graft site and to provide a conceptual framework for the rational design of cell matrix composite grafts.Keywords
This publication has 70 references indexed in Scilit:
- Osteoprogenitor cells within skeletal muscleJournal of Orthopaedic Research, 2000
- Tissue Engineering of BonePublished by Ovid Technologies (Wolters Kluwer Health) ,1999
- Uno, nessuno e centomila: Searching for the Identity of Mesodermal ProgenitorsExperimental Cell Research, 1999
- A Recombinant Human TGF-β1 Fusion Protein with Collagen-Binding Domain Promotes Migration, Growth, and Differentiation of Bone Marrow Mesenchymal CellsExperimental Cell Research, 1999
- Osteoprogenitor cell frequency in rat bone marrow stromal populations: Role for heterotypic cell-cell interactions in osteoblast differentiationJournal of Cellular Biochemistry, 1999
- Advances in the osteoblast lineageBiochemistry and Cell Biology, 1998
- Confocal images of marrow stromal (Westen-Bainton) cellsHistochemistry and Cell Biology, 1993
- Marrow stromal (Westen-Bainton) cells: Identification, morphometry, confocal imaging and changes in diseaseBone, 1993
- Animal Model for Evaluating Bone Repair with and without Adjunctive Hyperbaric Oxygen Therapy (HBO): Comparing Dose SchedulesJournal of Investigative Surgery, 1990
- Influence of Oxygen Concentration and Mechanical Factors on Differentiation of Connective Tissues in vitroNature, 1961