Venezuelan Equine Encephalomyelitis Virus Structure and Its Divergence from Old World Alphaviruses
Open Access
- 1 October 2001
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 75 (19), 9532-9537
- https://doi.org/10.1128/jvi.75.19.9532-9537.2001
Abstract
Although alphaviruses have been extensively studied as model systems for the structural organization of enveloped viruses, no structures exist for the phylogenetically distinct eastern equine encephalomyelitis (EEE)-Venezuelan equine encephalomyelitis (VEE) lineage of New World alphaviruses. Here we report the 25-Åstructure of VEE virus, obtained from electron cryomicroscopy and image reconstruction. The envelope spike glycoproteins of VEE virus have a T=4 icosahedral arrangement, similar to that observed in Old World Sindbis, Semliki Forest, and Ross River alphaviruses. However, VEE virus has pronounced differences in its nucleocapsid structure relative to nucleocapsid structures repeatedly observed in Old World alphaviruses.This publication has 19 references indexed in Scilit:
- Cryo-Electron Microscopy Reveals the Functional Organization of an Enveloped Virus, Semliki Forest VirusMolecular Cell, 2000
- Recombinational history and molecular evolution of western equine encephalomyelitis complex alphaviruses.1997
- Nucleocapsid and glycoprotein organization in an enveloped virusCell, 1995
- THE ALPHAVIRUSES - GENE-EXPRESSION, REPLICATION, AND EVOLUTION1994
- Three-dimensional structure of a membrane-containing virus.Proceedings of the National Academy of Sciences of the United States of America, 1993
- Spike protein-nucleocapsid interactions drive the budding of alphaviruses.1992
- The cytoplasmic domain of alphavirus E2 glycoprotein contains a short linear recognition signal required for viral budding.1991
- The full-length nucleotide sequences of the virulent Trinidad donkey strain of Venezuelan equine encephalitis virus and its attenuated vaccine derivative, strain TC-83Virology, 1989
- The T=4 envelope of sindbis virus is organized by interactions with a complementary T=3 capsidCell, 1987
- Envelope structure of Semliki Forest virus reconstructed from cryo-electron micrographsNature, 1986