FSL J1-208, a Virulent Uncommon Phylogenetic Lineage IV Listeria monocytogenes Strain with a Small Chromosome Size and a Putative Virulence Plasmid Carrying Internalin-Like Genes
Open Access
- 15 March 2012
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 78 (6), 1876-1889
- https://doi.org/10.1128/aem.06969-11
Abstract
The bacterial genus Listeria contains both saprotrophic and facultative pathogenic species. A small genome size has been suggested to be associated with the loss of pathogenic potential of L. welshimeri and L. seeligeri . In this paper we present data on the genome of L. monocytogenes strain FSL J1-208, a representative of phylogenetic lineage IV. Although this strain was isolated from a clinical case in a caprine host and has no decreased invasiveness in human intestinal epithelial cells, our analyses show that this strain has one of the smallest Listeria chromosomes reported to date (2.78 Mb). The chromosome contains 2,772 protein-coding genes, including well-characterized virulence-associated genes, such as inlA , inlB , and inlC and the full prfA gene cluster. The small genome size is mainly caused by the absence of prophages in the genome of L. monocytogenes FSL J1-208, and further analyses showed that the total size of prophage-related regions is highly correlated to chromosome size in the genus Listeria. L. monocytogenes FSL J1-208 carries a unique type of plasmid of approximately 80 kbp that does not carry genes annotated as being involved in resistance to antibiotics or heavy metals. The accessory genes in this plasmid belong to the internalin family, a family of virulence-associated proteins, and therefore this is the first report of a potential virulence plasmid in the genus Listeria .Keywords
This publication has 56 references indexed in Scilit:
- Correlations Between Bacterial Ecology and Mobile DNACurrent Microbiology, 2010
- TranslatorX: multiple alignment of nucleotide sequences guided by amino acid translationsNucleic Acids Research, 2010
- What traits are carried on mobile genetic elements, and why?Heredity, 2010
- Hamiltonella defensa , genome evolution of protective bacterial endosymbiont from pathogenic ancestorsProceedings of the National Academy of Sciences of the United States of America, 2009
- IslandViewer: an integrated interface for computational identification and visualization of genomic islandsBioinformatics, 2009
- jModelTest: Phylogenetic Model AveragingMolecular Biology and Evolution, 2008
- Recent developments in the MAFFT multiple sequence alignment programBriefings in Bioinformatics, 2008
- Prophinder: a computational tool for prophage prediction in prokaryotic genomesBioinformatics, 2008
- RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed modelsBioinformatics, 2006
- The complete genome sequence ofLactobacillus bulgaricusreveals extensive and ongoing reductive evolutionProceedings of the National Academy of Sciences of the United States of America, 2006