Mutagenicity testing of certified food colors and related azo, xanthene and triphenylmethane dyes with the Salmonella/microsome system
- 1 January 1978
- journal article
- research article
- Published by Elsevier BV in Mutation Research
- Vol. 56 (3), 249-271
- https://doi.org/10.1016/0027-5107(78)90192-6
Abstract
Thirty-seven azo, xanthene and triphenylmethane dyes, including FD and C colors currently approved for use in the U.S.A. and a number of delisted food colors, were tested in the Salmonella/microsome system. In addition to direct plate tests with five tester strains (TA1535, TA100, TA1537, TA1538, TA98), the azo dyes were also assayed after chemical reduction to their component amines. Also, a selected group of azo dyes was subjected to liquid tests (both aerobic with microsomes and anaerobic) and to plate tests involving initial 16 h anaerobic incubations to facilitate microbial reduction of the azo bond. None of the presently listed FD and C colors was mutagenic in any of the test modifications. Among formerly listed colors only Butter Yellow (p-dimethylaminoazonbenzen), a recognized animal carcinogen, was mutagenic in the aerobic liquid test. Several other azo dyes were either directly mutagenic, viz. Acid Alizarin Yellow R and Alizarin Yellow GG; required microsomal activation, viz. Acid Alizarin Red B and Methyl Red; or required chemical reduction and microsomal activation, viz. Acid Alizarin Violet N and Sudan IV. Of the non-azo dyes tested only two xanthene dyes appeared to be mutagenic, viz. 9-(2-sulfophenyl)-6-hydroxy-3-isoxanthenone and its 2,4,5,7-tetrabromo derivative.This publication has 17 references indexed in Scilit:
- Antitrichomonad Action, Mutagenicity, and Reduction of Metronidazole and Other NitroimidazolesAntimicrobial Agents and Chemotherapy, 1976
- Mutagenesis by 9,10-anthraquinone derivatives and related compounds in Salmonella typhimuriumMutation Research/Genetic Toxicology, 1976
- Methods for detecting carcinogens and mutagens with the salmonella/mammalian-microsome mutagenicity testMutation Research/Environmental Mutagenesis and Related Subjects, 1975
- Metabolic Reduction of Benzidine Azo Dyes to Benzidine in the Rhesus Monkey2JNCI Journal of the National Cancer Institute, 1975
- Toxicology of Food ColorsAnnual Review of Pharmacology, 1974
- Use of anaerobic glove boxes for the cultivation of strictly anaerobic bacteriaThe American Journal of Clinical Nutrition, 1972
- In vitro and host-mediated “rec-assay” procedures for screening chemical mutagens; and phloxine, a mutagenic red dye detectedMutation Research, 1972
- Mechanisms of Azo Reduction byStreptococcus faecalisII. The Role of Soluble FlavinsXenobiotica, 1971
- The Metabolic Activation of Carcinogenic Aromatic Amines and Amides1Published by S. Karger AG ,1969
- Studies on the mechanism of action of mammalian hepatic azoreductase—IBiochemical Pharmacology, 1967