Plasma membrane polarization during mating in yeast cells
Open Access
- 12 June 2006
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 173 (6), 861-866
- https://doi.org/10.1083/jcb.200602007
Abstract
The yeast mating cell provides a simple paradigm for analyzing mechanisms underlying the generation of surface polarity. Endocytic recycling and slow diffusion on the plasma membrane were shown to facilitate polarized surface distribution of Snc1p (Valdez-Taubas, J., and H.R. Pelham. 2003. Curr. Biol. 13:1636–1640). Here, we found that polarization of Fus1p, a raft-associated type I transmembrane protein involved in cell fusion, does not depend on endocytosis. Instead, Fus1p localization to the tip of the mating projection was determined by its cytosolic domain, which binds to peripheral proteins involved in mating tip polarization. Furthermore, we provide evidence that the lipid bilayer at the mating projection is more condensed than the plasma membrane enclosing the cell body, and that sphingolipids are required for this lipid organization.Keywords
This publication has 32 references indexed in Scilit:
- FUS1Regulates the Opening and Expansion of Fusion Pores between Mating YeastMolecular Biology of the Cell, 2006
- Lipids as Modulators of Proteolytic Activity of BACEPublished by Elsevier BV ,2005
- A Membrane Binding Domain in the Ste5 Scaffold Synergizes with Gβγ Binding to Control Localization and Signaling in Pheromone ResponseMolecular Cell, 2005
- Condensation of the plasma membrane at the site of T lymphocyte activationThe Journal of cell biology, 2005
- Exogenous Ganglioside GD1a Enhances Epidermal Growth Factor Receptor Binding and DimerizationJournal of Biological Chemistry, 2004
- Slow Diffusion of Proteins in the Yeast Plasma Membrane Allows Polarity to Be Maintained by Endocytic CyclingCurrent Biology, 2003
- A Combined Experimental and Computational Strategy to Define Protein Interaction Networks for Peptide Recognition ModulesScience, 2002
- Plasma Membrane Compartmentalization in Yeast by Messenger RNA Transport and a Septin Diffusion BarrierScience, 2000
- CELL POLARITY AND MORPHOGENESIS IN BUDDING YEASTAnnual Review of Microbiology, 1998
- Origins of Cell PolarityCell, 1996