An R2R3 MYB transcription factor associated with regulation of the anthocyanin biosynthetic pathway in Rosaceae
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Open Access
- 21 March 2010
- journal article
- Published by Springer Science and Business Media LLC in BMC Plant Biology
- Vol. 10 (1), 50
- https://doi.org/10.1186/1471-2229-10-50
Abstract
The control of plant anthocyanin accumulation is via transcriptional regulation of the genes encoding the biosynthetic enzymes. A key activator appears to be an R2R3 MYB transcription factor. In apple fruit, skin anthocyanin levels are controlled by a gene called MYBA or MYB1, while the gene determining fruit flesh and foliage anthocyanin has been termed MYB10. In order to further understand tissue-specific anthocyanin regulation we have isolated orthologous MYB genes from all the commercially important rosaceous species. We use gene specific primers to show that the three MYB activators of apple anthocyanin (MYB10/MYB1/MYBA) are likely alleles of each other. MYB transcription factors, with high sequence identity to the apple gene were isolated from across the rosaceous family (e.g. apples, pears, plums, cherries, peaches, raspberries, rose, strawberry). Key identifying amino acid residues were found in both the DNA-binding and C-terminal domains of these MYBs. The expression of these MYB10 genes correlates with fruit and flower anthocyanin levels. Their function was tested in tobacco and strawberry. In tobacco, these MYBs were shown to induce the anthocyanin pathway when co-expressed with bHLHs, while over-expression of strawberry and apple genes in the crop of origin elevates anthocyanins. This family-wide study of rosaceous R2R3 MYBs provides insight into the evolution of this plant trait. It has implications for the development of new coloured fruit and flowers, as well as aiding the understanding of temporal-spatial colour change.Keywords
This publication has 81 references indexed in Scilit:
- Multiple Repeats of a Promoter Segment Causes Transcription Factor Autoregulation in Red ApplesPlant Cell, 2009
- The Transcript and Metabolite Networks Affected by the Two Clades of Arabidopsis Glucosinolate Biosynthesis RegulatorsPlant Physiology, 2008
- TheArabidopsisTranscription Factor MYB77 Modulates Auxin Signal TransductionPlant Cell, 2007
- Red colouration in apple fruit is due to the activity of the MYB transcription factor, MdMYB10The Plant Journal, 2007
- Isolation of a Regulatory Gene of Anthocyanin Biosynthesis in Tuberous Roots of Purple-Fleshed Sweet PotatoPlant Physiology, 2007
- The Grapevine Transcription Factor VvMYBPA1 Regulates Proanthocyanidin Synthesis during Fruit DevelopmentPlant Physiology, 2007
- Light-Induced Expression of a MYB Gene Regulates Anthocyanin Biosynthesis in Red ApplesPlant Physiology, 2006
- PH4 of Petunia Is an R2R3 MYB Protein That Activates Vacuolar Acidification through Interactions with Basic-Helix-Loop-Helix Transcription Factors of the Anthocyanin PathwayPlant Cell, 2006
- NewAgrobacterium helper plasmids for gene transfer to plantsTransgenic Research, 1993
- A simple and efficient method for isolating RNA from pine treesPlant Molecular Biology Reporter, 1993