Multiplexed Sandwich Immunoassays Using Electrochemiluminescence Imaging Resolved at the Single Bead Level
- 10 April 2009
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 131 (17), 6088-6089
- https://doi.org/10.1021/ja901876z
Abstract
A new class of bead-based microarray that uses electrogenerated chemiluminescence (ECL) as a readout mechanism to detect multiple antigens simultaneously is presented. This platform demonstrates the possibility of performing highly multiplexed assays using ECL because all the individual sensing beads in the array are simultaneously imaged and individually resolved by ECL. Duplex and triplex assay results are demonstrated as well as a cross reactivity study.Keywords
This publication has 13 references indexed in Scilit:
- Fiber-Optic Microsphere-Based Antibody Array for the Analysis of Inflammatory Cytokines in SalivaAnalytical Chemistry, 2009
- Electrogenerated Chemiluminescence and Its Biorelated ApplicationsChemical Reviews, 2008
- Observing Single Nanoparticle Collisions by Electrogenerated Chemiluminescence AmplificationNano Letters, 2008
- Electrochemiluminescent Arrays for Cytochrome P450-Activated Genotoxicity Screening. DNA Damage from Benzo[a]pyrene MetabolitesAnalytical Chemistry, 2007
- Electrogenerated Chemiluminescence. 77. DNA Hybridization Detection at High Amplification with [Ru(bpy)3]2+-Containing MicrospheresAnalytical Chemistry, 2004
- Electrochemiluminescence (ECL)Chemical Reviews, 2004
- Bioactive Protein Nanoarrays on Nickel Oxide Surfaces Formed by Dip-Pen NanolithographyAngewandte Chemie, 2004
- Electrogenerated Chemiluminescence 69: The Tris(2,2‘-bipyridine)ruthenium(II), (Ru(bpy)32+)/Tri-n-propylamine (TPrA) System RevisitedA New Route Involving TPrA•+ Cation RadicalsJournal of the American Chemical Society, 2002
- A new non-isotopic detection system for immunoassaysNature, 1995
- Electrochemiluminescence: A New Diagnostic and Research ToolNature Biotechnology, 1994