Isolation and partial characterization of a protease involved in Fas‐induced apoptosis
- 8 May 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 364 (2), 139-142
- https://doi.org/10.1016/0014-5793(95)00374-i
Abstract
Protease involvement has been implicated in the signalling process of activation‐induced apoptosis. Here we report the isolation of a protease from Jurkat T cells undergoing Fas‐induced apoptosis. Although the protease probably is a serine protease, it seems to be distantly related to members of the ICE/ced‐3/Ich‐1(nedd‐2) family. In a cell‐free system using isolated thymocyte nuclei, the protease rapidly induces DNA fragmentation and morphological changes typically seen in apoptosis. Our results clearly show protease activation downstream to Fas‐ligation and implicate an important role for the isolated protease in signalling of Fas‐induced apoptosis.Keywords
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