Pluronic/chitosan hydrogels containing epidermal growth factor with wound‐adhesive and photo‐crosslinkable properties
- 19 August 2010
- journal article
- research article
- Published by Wiley in Journal of Biomedical Materials Research Part A
- Vol. 95A (2), 564-573
- https://doi.org/10.1002/jbm.a.32848
Abstract
Wound‐adhesive and thermo‐responsive hydrogels were prepared in an aim to develop a wound‐care device for diabetic ulcers. A mixture of glycidyl methacrylated chitooligosaccharide (COS), di‐acrylated Pluronic, and a recombinant human epidermal growth factor (rhEGF) formed a physical hydrogel. Photo‐irradiation was subsequently applied to chemically crosslink the hydrogel. Release profiles of encapsulated rhEGF from the hydrogel showed that release rates were dependent on degradation rates of hydrogels. Human primary keratinocytes were cultivated with the released fraction from the hydrogel to measure effects of released rhEGF on in vitro differentiation of keratinocytes. Muco‐adhesive property of the hydrogel was investigated in animal skins and significant amounts of quantum dot‐labeled rhEGF were retained at wound sites along with chitosan. The hydrogel was administered to dorsal burn wounds in wound healing impaired animals and photo‐irradiated. COS and rhEGF in the hydrogels significantly enhanced epidermal differentiation during the wound healing process. Thus, the rhEGF‐encapsulated hydrogel was expected to be a potential wound care product by increasing local concentration of rhEGF at wound sites with maintaining keratinocytic differentiation. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.Keywords
This publication has 37 references indexed in Scilit:
- Cultured keratinocytes in fibrin with decellularised dermis close porcine full-thickness wounds in a single stepBurns, 2008
- Insulin-like growth factor-I in wound healing of rat skinRegulatory Peptides, 2008
- Synthesis and properties of waterborne polyurethane hydrogels for wound healing dressingsJournal of Biomedical Materials Research Part B: Applied Biomaterials, 2007
- Controlled-release of epidermal growth factor from cationized gelatin hydrogel enhances corneal epithelial wound healingJournal of Controlled Release, 2006
- An investigation on burn wound healing in rats with chitosan gel formulation containing epidermal growth factorBurns, 2006
- Delivery of basic fibroblast growth factor (bFGF) from photoresponsive hydrogel scaffoldsBiomaterials, 2006
- Evaluation of the effect of incorporation of dibutyryl cyclic adenosine monophosphate in an in situ-forming hydrogel wound dressing based on oxidized alginate and gelatinBiomaterials, 2006
- Photocrosslinkable chitosan hydrogel containing fibroblast growth factor-2 stimulates wound healing in healing-impaired db/db miceBiomaterials, 2003
- Interactions of cytokines, growth factors, and proteases in acute and chronic woundsWound Repair and Regeneration, 1996
- Acidic Fibroblast Growth Factor Accelerates Dermal Wound Healing in Diabetic MiceJournal of Investigative Dermatology, 1995