Derivation of human embryonic stem cells in defined conditions
Top Cited Papers
- 1 January 2006
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Biotechnology
- Vol. 24 (2), 185-187
- https://doi.org/10.1038/nbt1177
Abstract
We have previously reported that high concentrations of basic fibroblast growth factor (bFGF) support feeder-independent growth of human embryonic stem (ES) cells, but those conditions included poorly defined serum and matrix components. Here we report feeder-independent human ES cell culture that includes protein components solely derived from recombinant sources or purified from human material. We describe the derivation of two new human ES cell lines in these defined culture conditions.Keywords
This publication has 15 references indexed in Scilit:
- Human embryonic stem cells derived without feeder cellsThe Lancet, 2005
- Basic Fibroblast Growth Factor Supports Undifferentiated Human Embryonic Stem Cell Growth Without Conditioned MediumThe International Journal of Cell Cloning, 2005
- Basic FGF and suppression of BMP signaling sustain undifferentiated proliferation of human ES cellsNature Methods, 2005
- Human embryonic stem cells express an immunogenic nonhuman sialic acidNature Medicine, 2005
- Gene expression patterns in human embryonic stem cells and human pluripotent germ cell tumorsProceedings of the National Academy of Sciences of the United States of America, 2003
- BMP Induction of Id Proteins Suppresses Differentiation and Sustains Embryonic Stem Cell Self-Renewal in Collaboration with STAT3Cell, 2003
- BMP4 initiates human embryonic stem cell differentiation to trophoblastNature Biotechnology, 2002
- Clonally Derived Human Embryonic Stem Cell Lines Maintain Pluripotency and Proliferative Potential for Prolonged Periods of CultureDevelopmental Biology, 2000
- Embryonic Stem Cell Lines Derived from Human BlastocystsScience, 1998
- A RAPID METHOD FOR VIABLE CELL TITRATION AND CLONE PRODUCTION WITH HELA CELLS IN TISSUE CULTURE: THE USE OF X-IRRADIATED CELLS TO SUPPLY CONDITIONING FACTORSProceedings of the National Academy of Sciences, 1955