Expression of Cell Proliferation-associated Nuclear Antigen (Ki-67) in Recurrent Aphthous Ulcers(RAU).
- 1 January 1998
- journal article
- Published by The Japanese Society for Oral Pathology in Oral Medicine & Pathology
- Vol. 3 (1), 29-34
- https://doi.org/10.3353/omp.3.29
Abstract
The histological features of recurrent aphthous ulcer (RAU) are consistent with increased number and activation of T cells in the diseased areas. A previous study suggested that 13-23% of the cells express IL-2 and transferrin receptors, which implies potential for local proliferation. The aim of the study was to to evaluate the proliferation activity in 10 RAU lesions compared to 9 oral traumatic ulcer (TU) lesions. Demonstration of proliferating cells was performed by application of affinity-purified rabbit anti-human Ki-67 antibodies in avidin-biotin-peroxidase complex (ABC) staining to both RAU and TU biopsies. We found that the basal/suprabasal layers of RAU epithelium away from the ulcer contained 30% more proliferating cells (127±24 vs 97±19 cells/0.2mm2, P2) of the infiltrating inflammatory cells in RAU lesions were Ki-67-positive proliferating cells, compared to only 3±1% (37±14 cells/0.2 mm2) in TU inflammatory infiltrates. These findings suggested that the mitotic activity is increased in RAU epithelium and that the relatively longer persistence of RAU cannot be explained by a lack of new epithelial cell production. Furthermore, mononuclear inflammatory cells replication in situ represent one mechanism for the expansion of the inflammatory cell infil-trates at sites of RAU and TU lesions.Keywords
This publication has 23 references indexed in Scilit:
- The Mechanism of Action of Cyclosporin A and FK506Clinical Immunology and Immunopathology, 1996
- Treatment of recurrent aphthous stomatitis with pentoxifyllineBritish Journal of Dermatology, 1995
- The injured cell: the role of the dendritic cell system as a sentinel receptor pathwayImmunology Today, 1995
- Tickling the TCR: selective T-cell functions stimulated by altered peptide ligandsImmunology Today, 1993
- Immune-activation in recurrent oral ulcers (ROU)European Journal of Oral Sciences, 1992
- Immune‐inflammatory cells in recurrent oral ulcers (ROU)European Journal of Oral Sciences, 1991
- Immunohistochemical markers of cellular proliferation: achievements, problems and prospectsCell Proliferation, 1990
- DNA‐synthesizing cells in oral epithelium have a range of cell cycle durations: evidence from double‐labelling studies using tritiated thymidine and bromodeoxyuridineCell Proliferation, 1989
- Allergic Contact Dermatitis: Novel Immunohistologic FeaturesJournal of Investigative Dermatology, 1986
- Association between recurrent aphthous ulcers and tobacco habitsEuropean Journal of Oral Sciences, 1985