Preformed reggie/flotillin caps: stable priming platforms for macrodomain assembly in T cells
Open Access
- 2 February 2006
- journal article
- Published by Wiley in The FASEB Journal
- Vol. 20 (6), 711-713
- https://doi.org/10.1096/fj.05-4760fje
Abstract
T cell activation after contact with an antigen-presenting cell depends on the regulated assembly of the T cell receptor signaling complex, which involves the polarized assembly of a stable, raft-like macrodomain surrounding engaged T cell receptors. Here we show that the preformed reggie/flotillin caps present in resting T cells act as priming platforms for macrodomain assembly. Preformed reggie-1/flotillin-2 caps are exceptionally stable, as shown by fluorescence recovery after photobleaching (FRAP). Upon T cell stimulation, signaling molecules are recruited to the stable reggie/flotillin caps. Importantly, a trans-negative reggie-1/flotillin-2 deletion mutant, which interferes with assembly of the preformed reggie/flotillin cap, impairs raft polarization and macrodomain formation after T cell activation. Accordingly, expression of the trans-negative reggie-1 mutant leads to the incorrect positioning of the guanine nucleotide exchange factor Vav, resulting in defects in cytoskeletal reorganization. Thus, the preformed reggie/flotillin caps are stable priming platforms for the assembly of multiprotein complexes controlling actin reorganization during T cell activation.Keywords
Funding Information
- Deutsche Forschungsgemeinschaft (SFB-TR11)
This publication has 58 references indexed in Scilit:
- Condensation of the plasma membrane at the site of T lymphocyte activationThe Journal of cell biology, 2005
- Detergent-resistant membranes should not be identified with membrane raftsTrends in Biochemical Sciences, 2005
- Merging complexes: properties of membrane raft assembly during lymphocyte signalingTrends in Immunology, 2005
- Ca2+ release via ryanodine receptors and Ca2+ entry: major mechanisms in NAADP-mediated Ca2+ signaling in T-lymphocytesCellular Signalling, 2004
- Membrane and raft association of reggie-1/flotillin-2: role of myristoylation, palmitoylation and oligomerization and induction of filopodia by overexpressionBiochemical Journal, 2004
- Lipid Rafts: Elusive or Illusive?Cell, 2003
- Signal Transduction Mediated by the T Cell Antigen Receptor: The Role of Adapter ProteinsAnnual Review of Immunology, 2002
- Vav1/Rac-dependent actin cytoskeleton reorganization is required for lipid raft clustering in T cellsThe Journal of cell biology, 2001
- Dynamic Actin Polymerization Drives T Cell Receptor–Induced Spreading: A Role for the Signal Transduction Adaptor LATImmunity, 2001
- Vav is a regulator of cytoskeletal reorganization mediated by the T-cell receptorCurrent Biology, 1998