Antiviral effect ofPhyllanthus nanus ethanolic extract against hepatitis B virus (HBV) by expression microarray analysis
- 19 October 2005
- journal article
- research article
- Published by Wiley in Journal of Cellular Biochemistry
- Vol. 97 (4), 795-812
- https://doi.org/10.1002/jcb.20611
Abstract
Ethanolic extract of Phyllanthus nanus (P. nanus) treatment exhibited potent antiviral activity against Hepatitis B virus (HBV). The effects of these extracts on HBV in the HBV genome integrated cell lines—Alexander cells and HepG2 2.2.15 cells were examined. Experimental results showed that the ethanolic extract of P. nanus produced suppressive effect on HBsAg secretion and HBsAg mRNA expression. The extract also inhibited HBV replication as measured by HBV DNA level in vitro. In addition, using a duck HBV (DHBV) primary culture model, the P. nanus ethanolic extract suppressed viral replication of DHBV in DHBV infected primary duck hepatocytes. The gene expression pattern in Alexander cells that had been treated with the ethanolic extract of P. nanus was also revealed by microarray techniques. The microarray results indicated that there was up-regulation of expression of several genes, including annexin A7 (Axn7). The subcellular localization of Axn7 and anti-HBV effect of Axn7 over-expression in Alexander cells were also investigated. Results showed that expression of Axn7–GFP fusion protein are localized around the secretory vesicles and could cause a decrease in HBsAg secretion in Alexander cells. Axn7 protein might play an important role in the medicinal effect of the active principle(s) of P. nanus. J. Cell. Biochem. 97: 795–812, 2006.Keywords
This publication has 62 references indexed in Scilit:
- Annexins and diseaseBiochemical and Biophysical Research Communications, 2004
- Infection of a human hepatoma cell line by hepatitis B virusProceedings of the National Academy of Sciences of the United States of America, 2002
- Quantitative Analysis of Hepatitis B Virus DNA by Real-Time AmplificationEuropean Journal of Clinical Microbiology & Infectious Diseases, 2001
- Activation of Annexin 7 by Protein Kinase C in Vitroand in VivoPublished by Elsevier BV ,2001
- Analysis of the mechanisms underlying the antinociceptive effect of the extracts of plants from the genus PhyllanthusGeneral Pharmacology: The Vascular System, 1995
- Characterization of cell lines established from human hepatocellular carcinomaInternational Journal of Cancer, 1995
- Annexins: the problem of assessing the biological role for a gene family of multifunctional calcium- and phospholipid-binding proteinsBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1994
- Simultaneous amplification and detection of specific hepatitis B virus and hepatitis C virus genomic sequences in serum samplesJournal of Medical Virology, 1994
- Production of hepatitis B virus by a differentiated human hepatoma cell line after transfection with cloned circular HBV DNACell, 1986
- Signals regulating hepatitis B surface antigen transcriptionNature, 1983