Co-assembly, spatiotemporal control and morphogenesis of a hybrid protein–peptide system
Open Access
- 28 September 2015
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Chemistry
- Vol. 7 (11), 897-904
- https://doi.org/10.1038/nchem.2349
Abstract
Controlling molecular interactions between bioinspired molecules can enable the development of new materials with higher complexity and innovative properties. Here we report on a dynamic system that emerges from the conformational modification of an elastin-like protein by peptide amphiphiles and with the capacity to access, and be maintained in, non-equilibrium for substantial periods of time. The system enables the formation of a robust membrane that displays controlled assembly and disassembly capabilities, adhesion and sealing to surfaces, self-healing and the capability to undergo morphogenesis into tubular structures with high spatiotemporal control. We use advanced microscopy along with turbidity and spectroscopic measurements to investigate the mechanism of assembly and its relation to the distinctive membrane architecture and the resulting dynamic properties. Using cell-culture experiments with endothelial and adipose-derived stem cells, we demonstrate the potential of this system to generate complex bioactive scaffolds for applications such as tissue engineering.Keywords
This publication has 41 references indexed in Scilit:
- Electrostatic Control of Structure in Self‐Assembled MembranesSmall, 2013
- How actin network dynamics control the onset of actin-based motilityProceedings of the National Academy of Sciences of the United States of America, 2012
- Cortactin Releases the Brakes in Actin- Based Motility by Enhancing WASP-VCA Detachment from Arp2/3 BranchesCurrent Biology, 2011
- A bioactive self-assembled membrane to promote angiogenesisBiomaterials, 2011
- Structural characterization of proteins and complexes using small-angle X-ray solution scatteringJournal of Structural Biology, 2010
- SDS-Induced Fibrillation of α-Synuclein: An Alternative Fibrillation PathwayJournal of Molecular Biology, 2010
- A self-assembly pathway to aligned monodomain gelsNature Materials, 2010
- Physical properties of hierarchically ordered self-assembled planar and spherical membranesSoft Matter, 2010
- The Role of Decorated SDS Micelles in Sub-CMC Protein Denaturation and AssociationJournal of Molecular Biology, 2009
- Pattern formation by local self‐activation and lateral inhibitionBioEssays, 2000