Use of heterologous antigens for the immunodiagnosis of abdominal angiostrongyliasis by an enzyme-linked immunosorbent assay.
Open Access
- 1 November 2010
- journal article
- Published by FapUNIFESP (SciELO) in Memórias do Instituto Oswaldo Cruz
- Vol. 105 (7), 914-917
- https://doi.org/10.1590/s0074-02762010000700013
Abstract
Angiostrongylus costaricensis has a broad geographic distribution spanning from North to South America and the infections of vertebrates with this nematode can result in abdominal complications. Human infections are diagnosed by histological or serological methods because the isolation of larvae from feces is not feasible, as most parasites become trapped in intestinal tissues due to intense eosinophilic inflammation. Because A. costaricensis is difficult to maintain in the laboratory, an immunodiagnostic IgG enzyme-linked immunosorbent assay (ELISA) using antigens from the congeneric Angiostrongylus cantonensis species was evaluated against a panel of serum samples from patients who were histologically diagnosed with A. costaricensis infections. Sera from uninfected individuals and individuals infected with other parasites were used as controls. The sensitivity and specificity of the assay were estimated at 88.4% and 78.7%, respectively. Because the use of purified or cloned antigens has not been established as a reliable diagnostic tool, the use of heterologous antigens may provide a viable alternative for the development of an ELISA-based immunodetection system for the diagnosis of abdominal angiostrongyliasis.Keywords
This publication has 21 references indexed in Scilit:
- Hydrophobic fractions from Strongyloides venezuelensis for use in the human immunodiagnosis of strongyloidiasisDiagnostic Microbiology and Infectious Disease, 2010
- Taenia saginata Metacestode Antigenic Fractions without Affinity to Concanavalin A Are an Important Source of Specific Antigens for the Diagnosis of Human NeurocysticercosisClinical and Vaccine Immunology, 2010
- Human angiostrongyliasisThe Lancet Infectious Diseases, 2008
- Angiostrongylus costaricensis egg antigen for the immunodiagnosis of abdominal angiostrongyliasisJournal of Helminthology, 2008
- IgG1, IgG4, and IgE antibody responses in human strongyloidiasis by ELISA using Strongyloides ratti saline extract as heterologous antigenZeitschrift für Parasitenkunde, 2007
- The expanding realm of heterologous immunity: friend or foe?Cellular Microbiology, 2006
- Caracterización de antígenos de bajo peso molecular de Angiostrongylus costaricensis, reconocidos durante una infección experimental en roedoresParasitología latinoamericana, 2004
- Seroepidemiology of abdominal angiostrongyliasis: the standardization of an immunoenzymatic assay and prevalence of antibodies in two localities in Southern BrazilTropical Medicine & International Health, 1997
- Angiostrongylus costaricensis: first record of its occurrence in the State of Espirito Santo, Brazil, and a review of its geographic distributionRevista do Instituto de Medicina Tropical de São Paulo, 1995
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976