Metabonomics: the use of electrospray mass spectrometry coupled to reversed‐phase liquid chromatography shows potential for the screening of rat urine in drug development
- 25 September 2002
- journal article
- research article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 16 (20), 1991-1996
- https://doi.org/10.1002/rcm.813
Abstract
The application of liquid chromatography/mass spectrometry (LC/MS) followed by principal components analysis (PCA) has been successfully applied to the screening of rat urine following the administration of three candidate pharmaceuticals. With this methodology it was possible to differentiate the control samples from the dosed samples and to identify the components of the mass spectrum responsible for the separation. These data clearly show that LC/MS is a viable alternative, or complementary, technique to proton NMR for metabonomics applications in drug discovery and development. Copyright © 2002 John Wiley & Sons, Ltd.Keywords
This publication has 15 references indexed in Scilit:
- Metabolic profiling of cell growth and death in cancer: applications in drug discoveryDrug Discovery Today, 2002
- The potential of serially coupled alkyl-bonded silica monolithic columns for high resolution separations of pharmaceutical compounds in biological fluidsChromatographia, 2002
- Metabonomics: a platform for studying drug toxicity and gene functionNature Reviews Drug Discovery, 2002
- Direct analysis of pharmaceutical compounds in human plasma with chromatographic resolution using an alkyl‐bonded silica rod columnRapid Communications in Mass Spectrometry, 2001
- Determination of 4‐hydroxytamoxifen in mouse plasma in the pg/mL range by gradient capillary liquid chromatography/tandem mass spectrometryRapid Communications in Mass Spectrometry, 2001
- An NMR‐based metabonomic approach to investigate the biochemical consequences of genetic strain differences: application to the C57BL10J and Alpk:ApfCD mouseFEBS Letters, 2000
- NMR spectroscopy based metabonomic studies on the comparative biochemistry of the kidney and urine of the bank vole (Clethrionomys glareolus), wood mouse (Apodemus sylvaticus), white toothed shrew (Crocidura suaveolens) and the laboratory ratComparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2000
- A metabonomic approach to the investigation of drug-induced phospholipidosis: an NMR spectroscopy and pattern recognition studyBiomarkers, 2000
- 'Metabonomics': understanding the metabolic responses of living systems to pathophysiological stimuli via multivariate statistical analysis of biological NMR spectroscopic dataXenobiotica, 1999
- Combined HPLC, NMR Spectroscopy, and Ion-Trap Mass Spectrometry with Application to the Detection and Characterization of Xenobiotic and Endogenous Metabolites in Human UrineAnalytical Chemistry, 1996