Effects of Fluid Shear Stress on a Distinct Population of Vascular Smooth Muscle Cells
- 5 October 2011
- journal article
- research article
- Published by Springer Science and Business Media LLC in Cellular and Molecular Bioengineering
- Vol. 4 (4), 627-636
- https://doi.org/10.1007/s12195-011-0205-8
Abstract
Vascular smooth muscle cells (SMCs) are a major cell type involved in vascular remodeling. The various developmental origins of SMCs such as neural crest and mesoderm result in the heterogeneity of SMCs, which plays an important role in vascular remodeling and disease development. Upon vascular injury, SMCs are exposed to blood flow and subjected to fluid shear stress. Previous studies have shown that fluid shear stress inhibits SMC proliferation. However, the effect of shear stress on the subpopulation of SMCs from specific developmental origin and vascular bed is not well understood. Here we investigated how shear stress regulates human aortic SMCs positive for neural crest markers. DNA microarray analysis showed that shear stress modulates the expression of genes involved in cell proliferation, matrix synthesis, cell signaling, transcription and cytoskeleton organization. Further studies demonstrated that shear stress induced SMC proliferation and cyclin D1, downregulated cell cycle inhibitor p21, and activated Akt pathway. Inhibition of PI-3 kinase blocked these shear stress-induced changes. These results suggest that SMCs with neural crest characteristics may respond to shear stress in a different manner. This finding has significant implications in the remodeling and disease development of blood vessels.Keywords
This publication has 37 references indexed in Scilit:
- PDGF-BB and TGF-β1 on cross-talk between endothelial and smooth muscle cells in vascular remodeling induced by low shear stressProceedings of the National Academy of Sciences of the United States of America, 2011
- Mechanisms of induction of endothelial cell E-selectin expression by smooth muscle cells and its inhibition by shear stressBlood, 2007
- Differential effects of equiaxial and uniaxial strain on mesenchymal stem cellsBiotechnology & Bioengineering, 2004
- The mTOR/p70 S6K1 pathway regulates vascular smooth muscle cell differentiationAmerican Journal of Physiology-Cell Physiology, 2004
- Smooth muscle stem cellsThe Anatomical Record Part A: Discoveries in Molecular, Cellular, and Evolutionary Biology, 2003
- Akt Controls Vascular Smooth Muscle Cell Proliferation In Vitro and In Vivo by Delaying G1/S ExitCirculation Research, 2003
- Involvement of PI3K/Akt pathway in cell cycle progression, apoptosis, and neoplastic transformation: a target for cancer chemotherapyLeukemia, 2003
- Distinct roles for the small GTPases Cdc42 and Rho in endothelial responses to shear stressJCI Insight, 1999
- Adenovirus-mediated over-expression of the cyclin/cyclin-dependent kinase inhibitor, p21 inhibits vascular smooth muscle cell proliferation and neointima formation in the rat carotid artery model of balloon angioplasty.JCI Insight, 1995
- The Role of Fluid Mechanics in the Localization and Detection of AtherosclerosisJournal of Biomechanical Engineering, 1993