Effect of nitrate on pea sucrose synthase
- 8 January 2009
- journal article
- Published by Pleiades Publishing Ltd in Russian Journal of Plant Physiology
- Vol. 56 (1), 74-79
- https://doi.org/10.1134/s1021443709010117
Abstract
The in vivo and in vitro nitrate effects on pea (Pisum sativum L.) sucrose synthase (SS) were studied. At the period of plant transition from heterotrophic to autotrophic nutrition, exogenous nitrate (14.2 mM) absorbed in the form of KNO3 and Ca(NO3)2 during 10–20 days activated SS in the roots by 22–100% as compared with plants grown on nitrogen-free medium. Such effect was observed only at plant growing under high light (natural illumination up to 25 klx) and thus their sufficient supplement with sucrose. Under low light (climate-controlled chamber, 2.5 klx), nitrate could not activate SS. In the in vitro experiments, nitrate activated SS exponentially by a dose-dependent mode with the plateau at 3–5 mM, where its activity was increased by 50%. It is supposed that there is a second constituent in SS activation by nitrate, and it carries information about plant carbohydrate status. Possible mechanisms of nitrate-induced SS activation are discussed.Keywords
This publication has 21 references indexed in Scilit:
- Activities of Sucrose Synthase and Acid Invertase in Pea Seedling OrgansRussian Journal of Plant Physiology, 2004
- LOCAL AND LONG-RANGE SIGNALING PATHWAYS REGULATING PLANT RESPONSES TO NITRATEAnnual Review of Plant Biology, 2002
- Short‐term metabolic responses of soybean root nodules to nitrateJournal of Experimental Botany, 2002
- Nitrogen and Carbon Nutrient and Metabolite Signaling in PlantsPlant Physiology, 2001
- Genomic Analysis of a Nutrient Response in Arabidopsis Reveals Diverse Expression Patterns and Novel Metabolic and Potential Regulatory Genes Induced by NitrateTHE PLANT CELL ONLINE, 2000
- Nitrate Acts as a Signal to Induce Organic Acid Metabolism and Repress Starch Metabolism in Tobacco.THE PLANT CELL ONLINE, 1997
- Nitrate: nutrient and signal for plant growth.THE PLANT CELL ONLINE, 1995
- Simultaneous Measurement of Intracellular pH and K+ or NO3- in Barley Root Cells Using Triple-Barreled, Ion-Selective MicroelectrodesPlant Physiology, 1995
- Sucrose synthase from wheat leaves. Comparison with the wheat germ enzymePhysiologia Plantarum, 1986
- Vacuoles as storage compartments for nitrate in barley leavesNature, 1981