Food Microbial Pathogen Detection and Analysis Using DNA Microarray Technologies
- 1 August 2008
- journal article
- research article
- Published by Mary Ann Liebert Inc in Foodborne Pathogens & Disease
- Vol. 5 (4), 531-550
- https://doi.org/10.1089/fpd.2008.0119
Abstract
Culture-based methods used for microbial detection and identification are simple to use, relatively inexpensive, and sensitive. However, culture-based methods are too time-consuming for high-throughput testing and too tedious for analysis of samples with multiple organisms and provide little clinical information regarding the pathogen (e.g., antibiotic resistance genes, virulence factors, or strain subtype). DNA-based methods, such as polymerase chain reaction (PCR), overcome some these limitations since they are generally faster and can provide more information than culture-based methods. One limitation of traditional PCR-based methods is that they are normally limited to the analysis of a single pathogen, a small group of related pathogens, or a small number of relevant genes. Microarray technology enables a significant expansion of the capability of DNA-based methods in terms of the number of DNA sequences that can be analyzed simultaneously, enabling molecular identification and characterization of multiple pathogens and many genes in a single array assay. Microarray analysis of microbial pathogens has potential uses in research, food safety, medical, agricultural, regulatory, public health, and industrial settings. In this article, we describe the main technical elements of microarray technology and the application and potential use of DNA microarrays for food microbial analysis.Keywords
This publication has 101 references indexed in Scilit:
- Use of a DNA Microarray for Simultaneous Detection of Antibiotic Resistance Genes among Staphylococcal Clinical IsolatesJournal of Clinical Microbiology, 2007
- Polymer Microfluidic Chips with Integrated Waveguides for Reading MicroarraysAnalytical Chemistry, 2007
- Impact of Feed Supplementation with Antimicrobial Agents on Growth Performance of Broiler Chickens,Clostridium perfringensandEnterococcusCounts, and Antibiotic Resistance Phenotypes and Distribution of Antimicrobial Resistance Determinants inEscherichia coliIsolatesApplied and Environmental Microbiology, 2007
- Luminex detection of fecal indicators in river samples, marine recreational water, and beach sandMarine Pollution Bulletin, 2007
- Label-free detection of biomolecules on microarrays using surface–colloid interactionAnalytical Biochemistry, 2007
- Development of a Serotype-Specific DNA Microarray for Identification of Some Shigella and Pathogenic Escherichia coli StrainsJournal of Clinical Microbiology, 2006
- Rapid Identification of Ascomycetous Yeasts from Clinical Specimens by a Molecular Method Based on Flow Cytometry and Comparison with Identifications from Phenotypic AssaysJournal of Clinical Microbiology, 2006
- Microarray and allele specific PCR detection of point mutations in Mycobacterium tuberculosis genes associated with drug resistanceJournal of Microbiological Methods, 2005
- Two Methods of Whole-Genome Amplification Enable Accurate Genotyping Across a 2320-SNP Linkage PanelGenome Research, 2004
- The Phylogeny and Signature Sequences Characteristics ofFibrobacteres,Chlorobi, andBacteroidetesCritical Reviews in Microbiology, 2004