Antioxidant and anticholinesterase activities of aqueous extract of Uraria picta (Jacq.) DC

Abstract

Numerous plants have been used in treating/managing Alzheimer’s disease in folklore. Inhibition of cholinesterase enzymes is an alternative way used in treating/managing Alzheimer’s disease. This study therefore sought to investigate the interaction of aqueous extract of Uraria picta with key enzymes (acetylcholinesterase and butyrylcholinesterase) linked with Alzheimer’s disease in vitro. Inhibition of acetylcholinesterase and butyrylcholinesterase, the total phenolic content and radical scavenging abilities were assessed in vitro. The extract inhibited acetylcholinesterase and butyrylcholinesterase in a dose dependent manner. Present in the extract are phenol and flavonoids. The extract also scavenged 2, 2 -azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) radical (ABTS*) and hydroxyl radical (OH*) in a dose-dependent manner. Inhibition of acetylcholinesterase, butyrylcholinesterase and the exhibited antioxidant properties could make U. picta extract a good means in treating/managing Alzheimer’s disease. Key words: Alzheimer’s disease, acetylcholinesterase, butyrylcholinesterase, Uraria picta, antioxidant.